Please wait a minute...
Acta Physico-Chimica Sinica  2010, Vol. 26 Issue (02): 359-366    DOI: 10.3866/PKU.WHXB20100207
CATALYSIS AND SURFACE STRUCTURE     
Adsorption Behavior and Mechanism of Grafted PMAA-HEMA/NVP Microspheres toward Lysozyme
FU Hong-Yan, GAO Bao-Jiao, NIU Qing-Yuan
Department of Chemical Engineering, North University of China, Taiyuan 030051, P. R. China
Download:   PDF(1288KB) Export: BibTeX | EndNote (RIS)      

Abstract  

The crosslinking copolymerization of 2-hydroxyethyl methacrylate (HEMA) and N-vinylpyrrolidone (NVP) was performed using the inverse suspension polymerization method resulting in crosslinked HEMA/NVP microspheres. Subsequently, the graft polymerization of methacrylic acid (MAA) was conducted using the“grafting from”method and the grafted PMAA-HEMA/NVP microspheres were obtained. The adsorption property and adsorption mechanism of the grafted microspheres were investigated in depth using lysozyme (LYZ) as a basic protein model. The zeta potential of the grafted microspheres was determined. Several factors that affected the adsorption property of the system such as the pH of the medium, the grafting degree of the microspheres and the ionic strength were examined. Experimental results indicate that in a wide pH range, the zeta potential of the grafted PMAA-HEMA/NVP microspheres has a large negative value. Therefore, a high density of negative charge is present on the surfaces of the grafted microspheres. The strong electrostatic interaction results in which the grafted PMAA-HEMA/NVP microspheres exhibit very strong adsorption ability toward lysozyme. The adsorption capacity of the grafted microspheres for lysozyme initially increases and then decreases as the pH increases and a maximumadsorption (90 mg·g-1) is found at pH=9, which is close to the isoelectric point of lysozyme. The ionic strength affects the adsorption property of the grafted microspheres greatly. As pH<9, the adsorption capacity of lysozyme decreases as the NaCl concentration increases, whereas as pH>9, the adsorption capacity of lysozyme increases as the NaCl concentration increases.



Key wordsPolymethacrylic acid      Grafted polymeric microspheres      Basic protein      Lysozyme      Adsorption mechanism     
Received: 22 July 2009      Published: 21 December 2009
MSC2000:  O647  
Corresponding Authors: GAO Bao-Jiao     E-mail: gaobaojiao@126.com
Cite this article:

FU Hong-Yan, GAO Bao-Jiao, NIU Qing-Yuan. Adsorption Behavior and Mechanism of Grafted PMAA-HEMA/NVP Microspheres toward Lysozyme. Acta Physico-Chimica Sinica, 2010, 26(02): 359-366.

URL:

http://www.whxb.pku.edu.cn/Jwk_wk/wlhx/10.3866/PKU.WHXB20100207     OR     http://www.whxb.pku.edu.cn/Jwk_wk/wlhx/Y2010/V26/I02/359

[1] SCHLERETH, Andrew NOOMUNA Panae, GAO Pei. Mesoscale Protein Patterning on a Self-Assembled Monolayer Coated Silicon Surface through Nanosphere Lithography[J]. Acta Physico-Chimica Sinica, 2017, 33(4): 810-815.
[2] JIAN Yuan, MU Wan-Jun, LIU Ning, PENG Shu-Ming. Removal of Sr2+ Ions by Ta-Doped Hexagonal WO3: Zeta Potential Measurements and Adsorption Mechanism Determination[J]. Acta Physico-Chimica Sinica, 2016, 32(8): 2052-2058.
[3] HONG Qi-Liang, DONG Yi-Hui, ZHUANG Wei, RAO Chao, LIU Chang. Kinetics and Thermodynamics of Lysozyme Adsorption on Mesoporous Titanium Dioxide[J]. Acta Physico-Chimica Sinica, 2016, 32(3): 638-646.
[4] LI Ya-Jing, SUN Xiao-Feng, YE Qing, LIU Bai-Chen, WU Yao-Guo. Preparation and Properties of a Novel Hemicellulose-Based Magnetic Hydrogel[J]. Acta Physico-Chimica Sinica, 2014, 30(1): 111-120.
[5] TANG Jing, ZENG Qiao, CHEN Zhen-Dong, HUANG Xiang-Qian. Electrochemical Indirect Measurement for the Study of Glutathione Adsorption on Multiwalled Carbon Nanotubes and Activated Carbon[J]. Acta Physico-Chimica Sinica, 2012, 28(05): 1269-1274.
[6] CAO Yin-Di, DANG Li-Qin, BAI Dan, LEI Zhi-Bin, LIU Mei-Ying. Adsorption of Lysozymes on Fe/CMK-5 Composites Synthesized by Chemical Vapor Deposition[J]. Acta Physico-Chimica Sinica, 2010, 26(06): 1593-1598.
[7] WANG Jian; GAO Bao-Jiao; GUO Hao-Peng. Adsorption Mechanism of Grafting Particle PMAA/SiO2 for Pirimicarb in Water Medium[J]. Acta Physico-Chimica Sinica, 2007, 23(12): 1905-1911.
[8] FANG Ying-Ying; HU Xin-Gen; YU Li; LI Wen-Bing; ZHU Yu-Qing; YU Sheng. Effect of DMSO on Denaturation of Hen Egg-white Lysozyme in Aqueous Solution[J]. Acta Physico-Chimica Sinica, 2007, 23(07): 1025-1031.
[9] FANG Ying-Ying;HU Xin-Gen;YU Li;LI Wen-Bing;LIN Rui-Sen. Study on Thermal Denaturation of Lysozyme by DSC[J]. Acta Physico-Chimica Sinica, 2007, 23(01): 84-87.
[10] Huang Jian-Gou;Xu Man-Cai;Li Hai-Tao;Shi Zuo-Qing;He Bing-Lin. Adsorption Thermodynamics of Macroporous Cross-linked Poly (N-methyl-N-p- vinylbenzylacetamine) Resin for Phenols from Non-aqueous System[J]. Acta Physico-Chimica Sinica, 2003, 19(03): 208-211.
[11] Dai Guo-Liang;Dong Xiang-Qun;Sun Zhi-Wei;Hu Wen-Rui. The Effect of Vapor Diffusion Rate on Lysozyme Crystal Growth[J]. Acta Physico-Chimica Sinica, 2002, 18(01): 70-73.
[12] Dai Guo-Liang;Dong Xiang-Qun;Sun Zhi-Wei;Hu Wen-Rui. Study on Lysozyme Crystal Growth by Vapor Diffusion Approach Using Dynamic Light Scattering Method[J]. Acta Physico-Chimica Sinica, 2001, 17(06): 531-536.
[13] Cao Ya, Li Hui-Lin, Zhang Ai-Min. Studies on Adsorption of Carboxy Methyl Cellulose Based Polymeric Surfactants at Solid/Liquid lnterface[J]. Acta Physico-Chimica Sinica, 1999, 15(10): 952-955.
[14] Miao Rui-Ping,Dai Min-Ping. Study of Activatied Carbon on the Adsorption of Titan Yellow from Aqueous Solution[J]. Acta Physico-Chimica Sinica, 1996, 12(02): 173-176.