ISSN 1000-6818CN 11-1892/O6CODEN WHXUEU
Acta Phys Chim Sin >> 2010,Vol.26>> Issue(10)>> 2672-2678     doi: 10.3866/PKU.WHXB20101003         中文摘要
Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy
DENG Lin, QI Zhi-Mei
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China
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We used time-resolved optical waveguide spectroscopy (OWGS) to in-situ measure visible absorption spectrum for methylene blue (MB) adsorbed ona hydrophilic glass waveguide from an aqueous solution containing proteins such as bovine serum albumin (BSA) and hemoglobin (Hb). The competitive adsorption of MB and the protein was detected at a solution pH lower than the isoelectric point of the protein. Since the competitive adsorption behavior of MB and the protein is very sensitive to the protein concentration, the protein content in the mixed solution was readily determined by time-resolved OWGS. In addition, a kinetic equation for the competitive adsorption of the two molecules was deduced based on the Langmuir adsorption isotherm. The best fit of the measured time course of the absorbance with the theoretical kinetic equation reveals that the number of MB molecules adsorbed on the surface exponentially decreases with time after reaching a maximum.A quasi-linear relationship between the fitting parameters and the protein concentration was also obtained.

Keywords: Optical waveguide spectroscopy   Competitive adsorption   Methylene blue   Protein detection  
Received: 2010-05-24 Accepted: 2010-07-05 Publication Date (Web): 2010-09-27
Corresponding Authors: QI Zhi-Mei Email:

Fund: The project was supported by the National Key Basic Research Programof China (973) (2009CB320300), National Natural Science Foundation of China (60978042), and the BaiRenJiHua Programof Chinese Academy of Sciences.

Cite this article: DENG Lin, QI Zhi-Mei. Study of Competitive Adsorption Behavior of Protein andMethylene Blue by Optical Waveguide Spectroscopy[J]. Acta Phys. -Chim. Sin., 2010,26 (10): 2672-2678.    doi: 10.3866/PKU.WHXB20101003

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