氨基吡啶修饰碳纳米管的制备及其与辣根过氧化酶的相互作用

doi:10.3866/PKU.WHXB20110125

Abstract

Abstract:

Carboxylic-functionalized multiwalled carbon nanotube (MWCNT-COOH) is obtained by oxidation with potassium bichromate and further modification by amide condensation afforded aminopyridine-grafted MWCNT (MWCNT-AP). The MWCNT-AP was characterized by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (¹H-NMR) and X-ray photoelectron spectroscopy (XPS). Transmission electron microscopy (TEM) results suggest that MWCNTCOOH aggregates in ethanol and that MWCNT-AP is stable and well dispersed in solution. Horseradish peroxidase (HRP) physically adsorbed onto the surfaces of MWCNT-AP and MWCNT-COOH and the adsorption amounts were 187.5 and 153.0 μg·mg^-1, respectively. UV-Vis spectra showed that the Soret band of HRP red-shifted markedly after adsorption onto MWCNT-AP or MWCNT-COOH indicating that the binding site of MWCNT-AP or MWCNT-COOH is near the heme pocket of HRP. Circular dichroism spectral results demonstrate that the secondary structure of HRP is influenced by MWCNT-AP. Enzyme-kinetic studies show that MWCNT-AP may adsorb HRP and its substrate 3,3',5,5'-tetramethylbenzidine (TMB) effectively, and the maximum reaction rate (V_max) of HRP increases significantly after interaction with MWCNT-AP.

Key words: Carbon nanotube, Surface functionalization, Horseradish peroxidase, Catalytic activity of enzyme, Hydrogen bonding

MSC2000:

O647

HAO Ping, GAO Yun-Yan, OU Zhi-Ze, LI Yi, WANG Zhong-Li, WANG Xue-Song. Preparation of Aminopyridine Grafted Carbon Nanotube and Its Interaction with Horseradish Peroxidase[J].Acta Phys. -Chim. Sin., 2011, 27(01): 233-240.

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Preparation of Aminopyridine Grafted Carbon Nanotube and Its Interaction with Horseradish Peroxidase

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