Register
ISSN 1000-6818CN 11-1892/O6CODEN WHXUEU
Acta Phys Chim Sin >> 2012,Vol.28>> Issue(06)>> 1520-1524     doi: 10.3866/PKU.WHXB201203131         中文摘要
PHYSICAL CHEMISTRY OF MATERIALS
Cell Biocompatibility of Functionalized Graphene Oxide
ZHANG Xiao1,2, YANG Rong2, WANG Chen2, HENG Cheng-Lin1
1. Key Laboratory of Cluster Science of Ministry of Education, School of Physics, Beijing Institute of Technology, Beijing 100081, P. R. China;
2. Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Chinese Academy of Sciences, National Center for Nanoscience and Technology, Beijing 100190, P. R. China
Full text: PDF (910KB) HTML Export: BibTeX | EndNote (RIS)
We report on synthesis of nanoscale graphene oxide (NGO) by modified Hummers’method. Synthesized NGO particles were surface functionalized by attaching carboxylic acid and polyethylene glycol groups to render them soluble in cell culture medium. The structures and properties of functionalized NGO were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and zeta potential analyzer. Cell viability studies show that PEG-modified NGO particles are highly soluble and incur almost no cytotoxicity to A549 cells, which suggest a great potential for the use of NGO in various biomedical applications.

Keywords: Graphene oxide   Nanomaterials   Biocompatibility   Surface functionalization  
Received: 2011-11-25 Accepted: 2012-03-11 Publication Date (Web): 2012-03-13
Corresponding Authors: YANG Rong, HENG Cheng-Lin Email: yangr@nanoctr.cn; hengcl@bit.edu.cn

Fund: The project was supported by the National Natural Science Foundation of China (20911130229, 21073047) and Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences, China (KJCX2.YW.M15).

Cite this article: ZHANG Xiao, YANG Rong, WANG Chen, HENG Cheng-Lin. Cell Biocompatibility of Functionalized Graphene Oxide[J]. Acta Phys. -Chim. Sin., 2012,28 (06): 1520-1524.    doi: 10.3866/PKU.WHXB201203131
(1) Geim, A. K.; Novoselov, K. S. Nat. Mater. 2007, 6, 183.  10.1038/nmat1849
(2) Rozhkov, A. V.; Giavaras, G.; Bliokh, Y. P.; Freilikher, V.; Nori, F. Phys. Rep. 2011, 503, 77.  10.1016/j.physrep.2011.02.002
(3) Novoselov, K. S.; Geim, A. K.; Morozov, S. V.; Jiang, D.; Zhang, Y.; Dubonos, S. V.; Grigorieva, I. V.; Firsov, A. A. Science 2004, 306, 666.  10.1126/science.1102896
(4) Xu, D.; Zhou, N. L.; Shen, J. Chem. J. Chin. U. 2010, 31 (12), 2354. [徐东, 周宁琳, 沈健. 高等学校化学学报, 2010, 31 (12), 2354.]
(5) Gu, X. G.; Yang, G.; Zhang, G. X.; Zhang, D. Q.; Zhu, D. B. ACS Appl. Mat. Interfaces 2011, 3, 1175.  10.1021/am2000104
(6) Schniepp, H. C.; Li, J. L.; McAllister, M. J.; Sai, H.; Herrera-Alonso, M.; Adamson, D. H.; Prud'homme, R. K.; Car, R.; Saville, D. A.; Aksay, I. A. J. Phys. Chem. B 2006, 110, 8535.
(7) Zhang, Q.; He, Y. Q.; Chen, X. G.; Hu, D. H.; Li, L. L.; Yi, T. Chin. Sci. Bull. 2010, 55, 620. [张琼, 贺蕴秋, 陈小刚, 胡栋虎, 李林江, 尹婷. 科学通报, 2010, 55, 620.]
(8) Liu, Y.; Yu, D. S.; Zeng, C.; Miao Z. C.; Dai, L. M. Langmuir 2010, 26, 6158.  10.1021/la100886x
(9) Yan, X. B.; Chen, J. T.; Yang, J.; Xue, Q. J.; Miele, P. ACS Appl. Mat. Interfaces 2010, 2, 2521.  10.1021/am100293r
(10) Zhang, L. M.; Xia, J. G.; Zhao, Q. H.; Zhang, Z. J. Small 2010, 4, 537.
(11) Yang, X. Y.; Zhang, X. Y.; Liu, Z. F.; Ma, Y. F.; Huang, Y.; Chen, Y. S. J. Phys. Chem. C 2008, 112, 17554.  10.1021/jp806751k
(12) Yang, K.; Zhang, S.; Zhang, G. X.; Sun, X. M.; Lee, S. T.; Liu, Z. Nano Lett. 2010, 10, 3318.  10.1021/nl100996u
(13) Chang, Y. L.; Yang, S. T.; Liu, J. H.; Dong, E.; Wang, Y. W.; Cao, A.; Liu, Y. F.; Wang, H. F. Toxicol. Lett. 2011, 200, 201.  10.1016/j.toxlet.2010.11.016
(14) Zhang, S.; Yang, K.; Feng, L. Z.; Liu, Z. Carbon, 2011, 49, 4040.  10.1016/j.carbon.2011.05.056
(15) Dikin, D. A.; Stankovich, S.; Zimney, E. J.; Piner, R. D.; Dommett, G. H. B.; Evmenenko, G.; Nguyen, S. T.; Ruoff, R. S. Nature 2007, 448, 457.  10.1038/nature06016
(16) Stankovich, S.; Dikin, D. A.; Dommett, G. H. B.; Kohlhaas, K. M.; Zimney, E. J.; Stach, E. A.; Piner, R. D.; Nguyen, S. T.; Ruoff, R. S. Nature 2006, 442, 282.  10.1038/nature04969
(17) Li, D.; Müller, M. B.; Gilje, S.; Kaner, R. B.; Wallace, G. G. Nat. Nanotechnol. 2008, 3, 101.  10.1038/nnano.2007.451
(18) Hummers, Jr. W. S.; Offeman, R. E. J. Am. Chem. Soc. 1958, 80, 1339.  10.1021/ja01539a017
(19)Hermanson, G. T. Bioconjugate techniques. http://www.sciencedirect.com/science/ book/9780123705013
(20) Fan, X. B.; Peng, W. C.; Li, Y.; Li, X. Y.; Wang, S. L.; Zhang, G. L.; Zhang, F. B. Adv. Mater 2008, 20, 4490.  10.1002/adma.200801306
(21) Rana, V. K.; Choi, M. C.; Kong, J. Y.; Kim, G. Y.; Kim, M. J.; Kim, S. H.; Mishra, S.; Singh, R. P.; Ha, C. S. Macromol. Mater. Eng. 2011, 296,131.  10.1002/mame.201000307
(22) Wang, G. X; Wang, B.; Park, J.; Yang, J.; Shen, X. P.; Yao, J. Carbon 2009, 47, 68.  10.1016/j.carbon.2008.09.002
(23) Shan, C. S.; Yang, H. F.; Han, D. X.; Zhang, Q. X.; Ivaska, A.; Niu, L. Langmuir 2009, 25, 12030.  10.1021/la903265p
(24) Si, Y. C.; Samulski, E. T. Nano Lett. 2008, 8, 1679.  10.1021/nl080604h
(25) Liu, Z.; Robinson, J. T.; Sun, X. M.; Dai, H. J. J. Am. Chem. Soc. 2008, 130, 10876.  10.1021/ja803688x
(26) Sun, X. M.; Liu, Z.; Welsher, K.; Robinson, J. T.; Goodwin, A.; Zaric, S.; Dai, H. J. Nano Res. 2008, 1, 203.  10.1007/s12274-008-8021-8
(27) Nguyen, T. T. T.; Tran, E.; Nguyen, T. H.; Do, P. T.; Huynh, T. H.; Huynh, H. Carcin. 2004, 25, 647.
(28) Wu, H. H.; Yang, R.; Song, B. M.; Han, Q. S.; Li, J. Y.; Zhang, Y.; Fang, Y.; Tenne, R. Wang, C. ACS Nano 2011, 5, 1276.  10.1021/nn102941b  
1. LIU Shan-Shan, XU Zheng, ZHAO Su-Ling, LIANG Zhi-Qin, ZHU Wei.Effects of F- Concentration on the Morphologies and Fluorescent Lifetimes of Ln3+-Doped Up-Conversion Nanocrystals[J]. Acta Phys. -Chim. Sin., 2016,32(8): 2108-2112
2. SUN Meng-Ting, HUANG Bi-Chun, MA Jie-Wen, LI Shi-Hui, DONG Li-Fu.Morphological Effects of Manganese Dioxide on Catalytic Reactions for Low-Temperature NH3-SCR[J]. Acta Phys. -Chim. Sin., 2016,32(6): 1501-1510
3. ZHAO Sheng-Jun, ZHANG Wei, DENG Hui-Ning, LIU Wei.Layer-by-Layer Assembly of Graphene Oxide and Polyelectrolyte Composite Membranes for Monovalent Cation Separation[J]. Acta Phys. -Chim. Sin., 2016,32(3): 723-727
4. FENG Lei, HAO Jing-Cheng.Preparation and Property of Gold Nanoparticles from Muliple Self- Assembled Structures as Templates in LA/C14DMAO/H2O System[J]. Acta Phys. -Chim. Sin., 2016,32(1): 380-390
5. JIAO Jin-Zhen, LI Shi-Hui, HUANG Bi-Chun.Preparation of Manganese Oxides Supported on Graphene Catalysts and Their Activity in Low-Temperature NH3-SCR[J]. Acta Phys. -Chim. Sin., 2015,31(7): 1383-1390
6. LIANG Yi, LU Yun, YAO Wei-Shang, ZHANG Xue-Tong.Polyimide Aerogels Crosslinked with Chemically Modified Graphene Oxide[J]. Acta Phys. -Chim. Sin., 2015,31(6): 1179-1185
7. XU Jing, YANG De-Zhi, LIAO Xiao-Zhen, HE Yu-Shi, MA Zi-Feng.Electrochemical Performances of Reduced Graphene Oxide/Titanium Dioxide Composites for Sodium-Ion Batteries[J]. Acta Phys. -Chim. Sin., 2015,31(5): 913-919
8. LI Wen-You, HE Yun-Qiu, LI Yi-Ming.Photoelectric Properties of Graphene Oxide Film Prepared with the Electrochemical Method Using Varying Levels of Reduction[J]. Acta Phys. -Chim. Sin., 2015,31(3): 457-466
9. YANG Jun-Li, WU Cong-Ling, LI Yuan-Hao, LI Wan-Li, MIAO Yan-Qin, GUO Kun-Peng, LIU Hui-Hui, WANG Hua, WU Yong-An.Effect of Graphene Oxide Doped PEDOT:PSS as a Hole Injection Layer on the Luminescence Performance of Organic Light-Emitting Diodes[J]. Acta Phys. -Chim. Sin., 2015,31(2): 377-383
10. MA Hui-Ling, ZHANG Long, ZHANG You-Wei, LIU Di, SUN Chao, ZENG Xin-Miao, ZHAI Mao-Lin.γ-Ray Induced Reduction of Graphene Oxide in Aqueous Solution[J]. Acta Phys. -Chim. Sin., 2015,31(10): 2016-2022
11. YU Chang-Lin, WEI Long-Fu, LI Jia-De, HE Hong-Bo, FANG Wen, ZHOU Wan-Qin.Preparation and Characterization of GO/Ag3PO4 Composite Photocatalyst and Its Visible Light Photocatalytic Performance[J]. Acta Phys. -Chim. Sin., 2015,31(10): 1932-1938
12. WANG Jian-De, PENG Tong-Jiang, XIAN Hai-Yang, SUN Hong-Juan.Preparation and Supercapacitive Performance of Three-Dimensional Reduced Graphene Oxide/Polyaniline Composite[J]. Acta Phys. -Chim. Sin., 2015,31(1): 90-98
13. WANG Li, MA Jun-Hong.Synthesis and Electrocatalytic Properties of Pt Nanoparticles on Nitrogen-Doped Reduced Graphene Oxide for Methanol Oxidation[J]. Acta Phys. -Chim. Sin., 2014,30(7): 1267-1273
14. WANG Xin-Huan, HAN Qiu-Sen, LI Jing-Ying, YANG Rong, DIAO Guo-Wang, WANG Chen.Seedless Synthesis of Gold Nanorods and Applications in Photo-Thermal Cancer Therapy[J]. Acta Phys. -Chim. Sin., 2014,30(7): 1363-1369
15. YANG Yu-Wen, FENG Gang, LU Zhang-Hui, HU Na, ZHANG Fei, CHEN Xiang-Shu.In situ Synthesis of Reduced Graphene Oxide Supported Co Nanoparticles as Efficient Catalysts for Hydrogen Generation from NH3BH3[J]. Acta Phys. -Chim. Sin., 2014,30(6): 1180-1186
16. LI Le, HE Yun-Qiu, CHU Xiao-Fei, LI Yi-Ming, SUN Fang-Fang, HUANG He-Zhou.Hydrothermal Synthesis of Partially Reduced Graphene Oxide-K2Mn4O8 Nanocomposites as Supercapacitors[J]. Acta Phys. -Chim. Sin., 2013,29(08): 1681-1690
17. SHI Qin, MEN Chun-Yan, LI Juan.Preparation and Electrochemical Capacitance Properties of Graphene Oxide/Polypyrrole Intercalation Composite[J]. Acta Phys. -Chim. Sin., 2013,29(08): 1691-1697
18. LONG Mei, CONG Ye, LI Xuan-Ke, CUI Zheng-Wei, DONG Zhi-Jun, YUAN Guan-Ming.Hydrothermal Synthesis and Photocatalytic Activity of Partially Reduced Graphene Oxide/TiO2 Composite[J]. Acta Phys. -Chim. Sin., 2013,29(06): 1344-1350
19. XU Jing, ZHU Yong-Fa.Elimination of Bisphenol A from Water via Graphene Oxide Adsorption[J]. Acta Phys. -Chim. Sin., 2013,29(04): 829-836
20. XU Gui-Yin, DING Bing, NIE Ping, LUO Hong-Jun, ZHANG Xiao-Gang.Preparation and Electrochemical Performance of Carbon Nanotubes/ Graphene Oxide/Sulfur Complex Cathode Material[J]. Acta Phys. -Chim. Sin., 2013,29(03): 546-552
21. DING Peng, XU You-Long, SUN Xiao-Fei.Synthesis and Performance of Nano MnO as an Anode Material for Lithium-Ion Batteries[J]. Acta Phys. -Chim. Sin., 2013,29(02): 293-297
22. ZHANG Hua, HU Yao-Juan, WU Ping, ZHANG Hui, CAI Chen-Xin.Preparation of an Ultrahigh Aspect Ratio Anodic Aluminum Oxide Template for the Fabrication of Ni Nanowire Arrays[J]. Acta Phys. -Chim. Sin., 2012,28(06): 1545-1550
23. CHEN Peng-Peng, WANG Jing, YAO Peng-Jun, DU Hai-Ying, LI Xiao-Gan.Synthesis and Gas Sensitivity of In2O3/CdO Composite[J]. Acta Phys. -Chim. Sin., 2012,28(06): 1539-1544
24. LI Xue-Fei, ZHAO Yun, JIAO Qing-Ze, LI Han-Sheng, WU Hong-Yu, LIU Hong-Bo, CUI Wen-Jia.Preparation of One-Dimensional Titanate Nanomaterials Using Different Titania Sources[J]. Acta Phys. -Chim. Sin., 2011,27(08): 1996-2000
25. MAI Li-Qiang, YANG Shuang, HAN Chun-Hua, XU Lin, XU Xu, PI Yu-Qiang.Chemical Lithiation and Electroactivity of Nanomaterials[J]. Acta Phys. -Chim. Sin., 2011,27(07): 1551-1559
26. SUN Du, YIN Peng-Gang, GUO Lin.Synthesis and Raman Property of Porous Jujube-Like Cu2O Hierarchy Structure[J]. Acta Phys. -Chim. Sin., 2011,27(06): 1543-1550
27. ZHANG Yi, CHEN Biao, YANG Zu-Pei, ZHANG Zhi-Jun.Controlled Synthesis and Characterization of the Structure and Property of Fe3O4 Nanoparticle-Graphene Oxide Composites[J]. Acta Phys. -Chim. Sin., 2011,27(05): 1261-1266
28. ZHAN Yong-Gong, CHEN Qi-Yuan, YIN Zhou-Lan, LI Li-Li, CAI Bing-Xin.Synthesis, Characterization and Surface Functionalization of Novel Spindle-Like α-Fe2O3 Nanocrystals[J]. Acta Phys. -Chim. Sin., 2010,26(11): 3113-3119
29. ZHAO Chuan-Xi, WU Ping, ZHANG Dan-Li, CHEN Rong, CHI Ling-Fei, XIAO Tan.Synthesis and Photoluminescence of Zn2SnO4 Nanomaterials[J]. Acta Phys. -Chim. Sin., 2010,26(05): 1343-1348
30. XU Can; ZHANG Xiao-Fang; CHEN Liang; ZHU Li-Fang; ZHANG Rong-Jun.Theoretical Study on the Even-Odd Oscillation of the Vibration Modes of the Silica Nanowire[J]. Acta Phys. -Chim. Sin., 2007,23(11): 1733-1737
31. SHE Shi-Xiong; SU Bi-Tao; YANG Yan; MIN Shi-Xiong; LEI Zi-Qiang.Preparation of Functional Polymer Nanomaterial and Its Catalytic Property[J]. Acta Phys. -Chim. Sin., 2007,23(06): 900-904
32. CHAI Yong-Cun;CHEN Xiao;SUI Zhen-Ming;ZHUANG Wen-Chang.Electrochemical Deposition of Bunchy Nanostructured Platinum from Lyotropic Liquid Crystal Template[J]. Acta Phys. -Chim. Sin., 2006,22(12): 1506-1510
33. WANG Zhong;TIAN Wen-Huai;LI Xing-Guo.Synthesis and Electrochemical Properties of Sn-Sb Alloy Prepared by Hydrogen Plasma-metal Reaction[J]. Acta Phys. -Chim. Sin., 2006,22(06): 752-755
34. CAO Jie-ming; WANG Jun; FANG Bao-qing; ZHENG Ming-bo; LU Hong-xia; CHANG Xin; WANG Hai-yan.Synthesis and Characterization of ZnO Nanomaterials with Different Morphologies in Ionic Liquids[J]. Acta Phys. -Chim. Sin., 2005,21(06): 668-672
35. Chen Feng; Zhu Yi-Ping; Ma Hong-Liao; Bo Zi-Long; Zhang Jin-Long.Preparation and Characterization of TiO2--CdS-MCM-41 Nano-composite[J]. Acta Phys. -Chim. Sin., 2004,20(11): 1292-1296
36. Li Di;Li Jing-Hong.Electrochemistry of Monolayer Protected Nanoclusters[J]. Acta Phys. -Chim. Sin., 2004,20(08S): 982-988
37. Sun Shi-Gang.Enhanced IR Absorption and Abnormal IR Effects of Low-dimensional Nanomaterials[J]. Acta Phys. -Chim. Sin., 2004,20(08S): 1017-1023
38. Du Jun;Wu Ling;Tao Chang-Yuan;Sun Cai-Xin.Preparation and Characterization of Fe3O4/PVDF Magnetic Composite Membrane[J]. Acta Phys. -Chim. Sin., 2004,20(06): 598-601
39. Wang Hui;Zhang Xiu-Juan;Zhang Xiao-Hong;Wu Shi-Kang.The Preparation of Nano-sized Silica Gel Suspension and the Recognition of Transition Metal Cations[J]. Acta Phys. -Chim. Sin., 2004,20(03): 313-317
40. Qiu Jie-Shan;An Yu-Liang;Li Qi-Xiu;Zhou Ying;Yang Qing.Preparation of Carbon Encapsulated Metal (Mn,Co) Nanomaterials with Apoferrintin as the Constrained Nano-sized Reactor[J]. Acta Phys. -Chim. Sin., 2004,20(03): 260-264
41. Dong Xiang-Ting;He Ying;Yan Jing-Hui;Xue Bo-Fei;Feng Xiu-Li;Hong Guang-Yan.Preparation and Characterization of Nanosized AgBr/PMMA Photochromic Hybrid Material[J]. Acta Phys. -Chim. Sin., 2003,19(12): 1159-1162
42. Duan Chun-Ying;Zhou Jing-Fang;Wu Zhi-Shen;Dang Hong-Xin.Preparation and Characterization of the Polystyrene/silver Core-shell Structure Nanospheres[J]. Acta Phys. -Chim. Sin., 2003,19(11): 1049-1053
43. Xu Jin-Xia;Huang Xin-Min;Qian Li-Hua.Structure and Properties of Ni-Al2O3 Nanochannel Array System by Direct Current Electro-deposition[J]. Acta Phys. -Chim. Sin., 2003,19(03): 265-267
44. Li Zhi-Wei;Zhou Jing-Fang;Zhang Zhi-Jun;Dang Hong-Xin.Preparation and Self-assembly on Glasses of Surface-functional Polystyrene Nanoparticles[J]. Acta Phys. -Chim. Sin., 2002,18(06): 550-553
45. Zhao Li-Li;Wang Rong-Shu;Niu Wen-Tai;Li Xiang.Synthesis of γ-MnO2 Nano Powder Used for SieveMembrane Reactor[J]. Acta Phys. -Chim. Sin., 2002,18(03): 197-200
46. Cao Da-Peng;Wang Wen-Chuan;Shen Zhi-Gang;Chen Jian-Feng.Determination of Optimum Pressure for Supercritical Methane Adsorbed in Nano-material[J]. Acta Phys. -Chim. Sin., 2001,17(10): 940-943
47. HOU Jing-Fei, YANG Yan-Lian, WANG Chen.Molecular Mechanisms of Interface Interactions between Nanomaterials and Proteins[J]. Acta Phys. -Chim. Sin., 0,(): 0-0
Copyright © 2006-2016 Editorial office of Acta Physico-Chimica Sinica
Address: College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P.R.China
Service Tel: +8610-62751724 Fax: +8610-62756388 Email:whxb@pku.edu.cn
^ Top