Acta Phys. -Chim. Sin. ›› 2012, Vol. 28 ›› Issue (06): 1502-1508.doi: 10.3866/PKU.WHXB201203201

• BIOPHYSICAL CHEMISTRY • Previous Articles     Next Articles

Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy

LI Mi1,2, LIU Lian-Qing1, XI Ning1,3, WANG Yue-Chao1, DONG Zai-Li1, XIAO Xiu-Bin4, ZHANG Wei-Jing4   

  1. 1. State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, P. R. China;
    2. Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
    3. Department of Electrical and Computer Engineering, Michigan State University, East Lansing 48824, USA;
    4. Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, P. R. China
  • Received:2012-03-09 Revised:2012-03-20 Published:2012-05-17
  • Contact: LIU Lian-Qing, XI Ning, ZHANG Wei-Jing E-mail:lqliu@sia.cn; xin@egr.msu.edu; zhangwj3072@163.com
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (60904095, 61175103), National High Technology Research and Development Program of China (863) (2009AA03Z316), and Chinese Academy of Sciences Foreign Experts Affairs International Partnership Program for Creative Research Teams.

Abstract: Atomic force microscopy (AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions. However, owing to the lack of adequate cellular immobilization methods, AFM imaging of living, suspended mammalian cells is still a big challenge. In this paper, a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine. In this way, the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically. When the cell is stimulated by 0.2 mg·mL-1 Rituximab for 2 h, the cell topography becomes more corrugated and Young's modulus decreases from 196 to 183 kPa. When the cell is stimulated by 0.5 mg·mL-1 Rituximab for 2 h, the cell topography changes more significantly and some tubercles appear, and Young's modulus decreases from 234 to 175 kPa. These results thus provide a unique insight into the effects of Rituximab on individual cells.

Key words: Atomic force microscopy, Lymphoma, Elasticity, Force curve, Young's modulus

MSC2000: 

  • O641