Please wait a minute...
Acta Phys. Chim. Sin.  2013, Vol. 29 Issue (07): 1588-1594    DOI: 10.3866/PKU.WHXB201304233
BIOPHYSICAL CHEMISTRY     
Preparation of Freestanding Bilayer Lipid Membranes and the Effect of Temperature on Their Mechanical Properties
WANG Yang, YAN Jia-Wei, ZHU Zai-Wen, ZHAO Xue-Qin, ZHONG Yun-Xing, MAO Bing-Wei
State Key Laboratory of Physical Chemistry of the Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, P. R. China
Download:   PDF(1466KB) Export: BibTeX | EndNote (RIS)      

Abstract  

Nanoporous silicon nitride membranes are fabricated by combining polystyrene colloidal lithography with Micro-Electro-Mechanical Systems, and then freestanding bilayer lipid membranes are constructed across the nanopores by the vesicle method. The topography and mechanical properties of the freestanding bilayer lipid membranes are investigated using the imaging and force curve modes of variable-temperature atomic force microscopy (AFM). The fluidity and self-repair of the freestanding bilayer lipid membranes are observed by AFM, which gives enough freestanding area for further investigations. Force curve measurements demonstrate that both breakthrough and adhesion forces decrease as the temperature increases; i.e., the mechanical stability of freestanding bilayer lipid membranes decreases as the temperature rises. The breakthrough force of freestanding bilayer lipid membranes is smaller than that of supported membranes. Moreover, the adhesion force of freestanding bilayer lipid membranes varies in the opposite manner to that of supported membranes.



Key wordsAtomic force microscopy      Freestanding bilayer lipid membrane      Nanoporous silicon nitride membrane      Vesicle      Temperature     
Received: 31 January 2013      Published: 23 April 2013
MSC2000:  O647  
Fund:  

The project was supported by the Natural Science Foundation of Fujian Province, China (2012J01054), National Natural Science Foundation of China (21021002), and Fundamental Research Funds for the Central Universities, China (2012121026).

Cite this article:

WANG Yang, YAN Jia-Wei, ZHU Zai-Wen, ZHAO Xue-Qin, ZHONG Yun-Xing, MAO Bing-Wei. Preparation of Freestanding Bilayer Lipid Membranes and the Effect of Temperature on Their Mechanical Properties. Acta Phys. Chim. Sin., 2013, 29(07): 1588-1594.

URL:

http://www.whxb.pku.edu.cn/Jwk_wk/wlhx/10.3866/PKU.WHXB201304233     OR     http://www.whxb.pku.edu.cn/Jwk_wk/wlhx/Y2013/V29/I07/1588

(1) Nielsen, C. H. Anal. Bioanal. Chem. 2009, 395, 697. doi: 10.1007/s00216-009-2960-0
(2) Ma,W. B.; Zhang, L. Z. Chem. Ind. Eng. Pro. 2007, 26, 1538.[马文宝, 张立志. 化工进展, 2007, 26, 1538.]
(3) Wang, L. G.; Li, Y. H.; Tien, H. T. Bioelectrochem. Bioenerg.1995, 36, 145.
(4) Yabuki, S.; Mizutani, F.; Hirata, Y. J. Electroanal. Chem. 1999,468, 117. doi: 10.1016/S0022-0728(99)00088-1
(5) Yang, C. Y.; Liu, Y.; Li, Q. G.; Li, L.W. Acta Phys. -Chim. Sin.2007, 23, 635. [杨昌英, 刘义, 李强国, 李林蔚. 物理化学学报, 2007, 23, 635.] doi: 10.1016/S1872-1508(07)60040-0
(6) Hao, C. C.; Sun, R. G.; Zhang, J. Chem. J. Chin. Univ. 2009, 30,913. [郝长春, 孙润广, 张静. 高等学校化学学报, 2009, 30,913.]
(7) Han, X. J.; Song, Y. H.;Wu, Z. Y.; Zhang, B. L.;Wang, E. K.Chem. J. Chin. Univ. 2001, 22, 1298. [韩晓军, 宋永海, 吴正岩, 张柏林, 汪尔康. 高等学校化学学报, 2001, 22, 1298.]
(8) Mabrey, S.; Sturtevant, J. M. Proc. Natl. Acad. Sci. 1976, 73,3862. doi: 10.1073/pnas.73.11.3862
(9) Janiak, M. J.; Small, D. M.; Shipley, G. G. J. Biol. Chem. 1979,254, 6068.
(10) Cheng, Y.; Bushby, R. J.; Evans, S. D.; Knowles, P. R.; Miles,R. E.; Ogier, S. D. Langmuir 2001, 17, 1240. doi: 10.1021/la001423h
(11) Steltenkamp, S.; Müller, M. M.; Deserno, M.; Hennesthal, C.;Steinem, C.; Janshoff, A. Biophys. J. 2006, 91, 217. doi: 10.1529/biophysj.106.081398
(12) Mey, I.; Stephan, M.; Schmitt, E. K.; Müller, M. M.; Ben Amar,M.; Steinem, C.; Janshoff, A. J. Am. Chem. Soc. 2009, 131,7031. doi: 10.1021/ja809165h
(13) Dufrene, Y. F.; Boland, T.; Schneider, J.W.; Barger,W. R.; Lee,G. U. Faraday Discuss. 1998, 111, 79.
(14) Dufrene, Y. F.; Barger,W. R.; Green, J. B.; Lee, G. U. Langmuir1997, 13, 4779. doi: 10.1021/la970221r
(15) Garcia-Manyes, S.; Lorena, R. M.; Gerard, O.; Sanz, F. J. Am. Chem. Soc. 2010, 132, 12874. doi: 10.1021/ja1002185
(16) Garcia-Manyes, S.; Oncins, G.; Sanz, F. Biophys. J. 2005, 89,1812. doi: 10.1529/biophysj.105.064030
(17) Garcia-Manyes, S.; Oncins, G.; Sanz, F. Biophys. J. 2005, 89,4261. doi: 10.1529/biophysj.105.065581
(18) Seeger, H. M.; Cerbo, A. D.; Alessandrini, A.; Facci, P. J. Phys. Chem. B 2010, 114, 8926. doi: 10.1021/jp1026477
(19) Keller, D.; Larsen, N. B.; Moller, I. M.; Mouritsen, O. G.Physical Review Lettlers 2005, 94, 025701. doi: 10.1103/PhysRevLett.94.025701
(20) Leonenko, Z. V.; Finot, E.; Ma, H.; Dahms, T. E.; Cramb, D. T.Biophys. J. 2004, 86, 3783. doi: 10.1529/biophysj.103.036681
(21) Seeger, H. M.; Marino, G.; Alessandrini, A.; Facci, P. Biophys. J. 2009, 97, 1067. doi: 10.1016/j.bpj.2009.03.068
(22) Charrier, A.; Thibaudau, F. Biophys. J. 2005, 89, 1094. doi: 10.1529/biophysj.105.062463
(23) Li, J.; Stein, D.; McMullan, C.; Branton, D.; Aziz, M. J.;Golovchenko, J. A. Nature 2001, 412, 166. doi: 10.1038/35084037
(24) Romer,W.; Steinem, C. Biophys. J. 2004, 86, 955.
(25) Zhang, X.; Zhu, Z.W.; Sun, C. F.; Zhu, F.; Luo, Z. Z.; Yan, J.W.; Mao, B.W. Microchim. Acta 2009, 167, 135. doi: 10.1007/s00604-009-0216-5
(26) Yu, Z.W.; Quinn, P. J. Biophys. J. 1995, 69, 1456.

[1] ZHAO Wen-Rong, HAO Jing-Cheng, Heinz Hoffmann. Vesicle Gels of Magnetic Asymmetric Surfactants[J]. Acta Phys. Chim. Sin., 2017, 33(8): 1655-1664.
[2] HUANG Xue-Hui, SHANG Xiao-Hui, NIU Peng-Ju. Surface Modification of SBA-15 and Its Effect on the Structure and Properties of Mesoporous La0.8Sr0.2CoO3[J]. Acta Phys. Chim. Sin., 2017, 33(7): 1462-1473.
[3] YUAN Kang, ZHOU Xue, DU Jian-Zhong. Synthesis and Characterization of Thermo-Responsive Polypeptide-Based Vesicles with Photo-Cross-Linked Membranes[J]. Acta Phys. Chim. Sin., 2017, 33(4): 656-660.
[4] SCHLERETH, Andrew NOOMUNA Panae, GAO Pei. Mesoscale Protein Patterning on a Self-Assembled Monolayer Coated Silicon Surface through Nanosphere Lithography[J]. Acta Phys. Chim. Sin., 2017, 33(4): 810-815.
[5] YAO Qian, PENG Li-Juan, LI Ze-Rong, LI Xiang-Yuan. Accurate Calculation of the Energy Barriers and Rate Constants of Hydrogen Abstraction from Alkanes by Hydroperoxyl Radical[J]. Acta Phys. Chim. Sin., 2017, 33(4): 763-768.
[6] GUO Rui, ZHANG Jialin, ZHAO Songtao, YU Xiaojiang, ZHONG Shu, SUN Shuo, LI Zhenyu, CHEN Wei. LT-STM Investigation of the Self-Assembled F16CuPc-Corannulene Binary System on Ag(111) and Grap[J]. Acta Phys. Chim. Sin., 2017, 33(3): 627-632.
[7] YIN Jin-Ling, LIU Jia, WEN Qing, WANG Gui-Ling, CAO Dian-Xue. Phosphomolybdic Acid as a Mediator for Indirect Carbon Electrooxidation in LowTemperature Carbon Fuel Cell[J]. Acta Phys. Chim. Sin., 2017, 33(2): 370-376.
[8] ZHANG Tao-Na, XU Xue-Wen, DONG Liang, TAN Zhao-Yi, LIU Chun-Li. Molecular Dynamics Simulations of Uranyl Species Adsorption and Diffusion Behavior on Pyrophyllite at Different Temperatures[J]. Acta Phys. Chim. Sin., 2017, 33(10): 2013-2021.
[9] LIU Meng-Xi, LI Shi-Chao, ZHA Ze-Qi, QIU Xiao-Hui. Research Progress and Applications of qPlus Noncontact Atomic Force Microscopy[J]. Acta Phys. Chim. Sin., 2017, 33(1): 183-197.
[10] ZHANG Wei-Feng, XIAN Lei-Yong, YONG Kang-Le, HE Jiu-Ning, ZHANG Chang-Hua, LI Ping, LI Xiang-Yuan. A Shock Tube Study of n-Undecane/Air Ignition Delays over a Wide Range of Temperatures[J]. Acta Phys. Chim. Sin., 2016, 32(9): 2216-2222.
[11] ZHANG Ji-Ping, CHENG Shuo-Zhen, LI Xue-Feng, DONG Jin-Feng. pH- and Temperature-Induced Micellization of the Dual Hydrophilic Block Copolymer Poly(methacrylate acid)-b-poly(N-(2-methacryloylxyethyl) pyrrolidone) in Aqueous Solution[J]. Acta Phys. Chim. Sin., 2016, 32(8): 2018-2026.
[12] WANG Jiao, YANG Li-Jun, ZHU Tian-Tian, WANG Shen-Zhi, CHEN Zhong-Xiu. Phase Transition of Phospholipid Vesicles Induced by Fatty Acids in Macromolecular Crowding: a Differential Scanning Calorimetry Study[J]. Acta Phys. Chim. Sin., 2016, 32(8): 2027-2038.
[13] YANG Zu-Guang, HUAWei-Bo, ZHANG Jun, CHEN Jiu-Hua, HE Feng-Rong, ZHONG Ben-He, GUO Xiao-Dong. Enhanced Electrochemical Performance of LiNi0.5Co0.2Mn0.3O2 Cathode Materials at Elevated Temperature by Zr Doping[J]. Acta Phys. Chim. Sin., 2016, 32(5): 1056-1061.
[14] CHEN Jian, WANG Xiao, LI Lei, ZENG Guang, SUN Peng, HUANG Wei. Radiation Response Behavior of a Diacetylene/Agarose Gel Dosimeter[J]. Acta Phys. Chim. Sin., 2016, 32(4): 1012-1018.
[15] HAN Chuan-Hong, GENG Pei-Pei, GUO Yan, CHEN Xiao-Xiao, GUO Xiao-Dong, ZHANG Jun-Hong, LIU Jie, WEI Xi-Lian. Thermoresponsive Properties of a Mixed Aqueous Solution of Cationic Surfactant and Organic Acid[J]. Acta Phys. Chim. Sin., 2016, 32(4): 863-871.