刷状结构的阳离子性接枝微粒QPDMAEMA/SiO2对牛血清白蛋白的吸附特性

doi:10.3866/PKU.WHXB201409151

Abstract

Abstract:

Cationic grafted particles with a brush structure were prepared with micron-sized silica gel particles as a matrix via graft-polymerization and macromolecular reaction. The adsorption ability, adsorption mechanism, and adsorption thermodynamics of bovine serum albumin (BSA) on the particles were investigated in depth. The tertiary amine group-containing monomer (dimethylaminoethyl methacrylate, DMAEMA) was first allowed to polymerize on the surfaces of silica gel particles by initiating the ―NH₂/S₂O₈_2- surface system, resulting in grafted PDMAEMA/SiO₂ particles. Subsequently, the tertiary amine groups in the chains of the grafted PDMAEMA macromolecules were quaternized with chlorethamin reagent to obtain the functional grafted QPDMAEMA/SiO₂ particles, on which the cationic polyelectrolyte QPDMAEMA macromolecules were grafted. The zeta potential of the QPDMAEMA/SiO₂ particles was determined to estimate their surface electrical property. Isothermal adsorption experiments were carried out to investigate the effects of several main factors, including the pH value of the medium, ion strength, and temperature, on the adsorption performance of QPDMAEMA/SiO₂ particles. Finally, the adsorption thermodynamics were investigated. The results showed that the functional grafted QPDMAEMA/SiO₂ particles had much higher zeta potential than PDMAEMA/SiO₂. BSA would be very strongly adsorbed on QPDMAEMA/SiO₂ particles through electrostatic interactions. The adsorption capacity first increased and then decreased with increasing pH value, and it had a maximum value of 112 mg·g^-1 when the pH value of the medium was equal to the isoelectric point of BSA (pI=4.7). On both sides of the isoelectric point, the effect of ion strength on the adsorption capacity was opposite. When the pH value of the medium was lower than the isoelectric point of BSA (i.e., pH<4.7), the adsorption capacity increased with increasing concentrations of electrolyte (NaCl). When the pH value of the medium was equal to the isoelectric point of BSA (i.e., pH=4.7), the adsorption capacity was almost unchanged with ion strength. The adsorption process was exothermic and during this process the entropy tended to decrease. Furthermore, this adsorption process was driven by enthalpy.

Key words: Polyelectrolyte brush, Bovine serum albumin, Dimethylaminoethyl methacrylate, Graft-polymerization, Electrostatic interaction, Adsorption

MSC2000:

O647

SHI Nan, GAO Bao-Jiao, YANG Qing. Adsorption Characteristics of Bovine Serum Albumin on Cationic Grafted Particles QPDMAEMA/SiO₂ with Brush Structure[J].Acta Phys. -Chim. Sin., 2014, 30(11): 2168-2176.

References

(1) Anirudhan, T. S.; Rejeena, S. R.; Tharun, A. R. Colloids Surf. BBiointerfaces 2012, 93, 49. doi: 10.1016/j.colsurfb.2011.12.010
(2) Jin, G.; Zhang, L.; Yao, Q. Z. J. Membr. Sci. 2007, 287, 271. doi: 10.1016/j.memsci.2006.10.047
(3) Kopac, T.; Bozgeyik, K.; Yener, J. Colloid Surf. A-Physicochem. Eng. Asp. 2008, 322, 19. doi: 10.1016/j.colsurfa.2008.02.010
(4) Yamasaki, K.; Chuang, V. T. G.; Maruyama, T.; Otagiri, M. Biochim. Biophys. Acta 2013, 1830, 5435. doi: 10.1016/j.bbagen.2013.05.005
(5) Dong, Y. S.; Zhang, F.;Wang, Z. M.; Du, L.; Hao, A. Y.; Jiang, B.; Tian, M. Y.; Li, Q.; Ji, Q. A.;Wang, S. C.; Xiu, Z. L. J. Chromatogr. A 2012, 1245, 143.
(6) Chen, Z.; He, Y.; Shi, B.; Yang, D. C. Biochim. Biophys. Acta 2013, 1830, 5515. doi: 10.1016/j.bbagen.2013.04.037
(7) Zhu, R. Y.; Xin, X.; Dai, H. Y.; Li, Q.; Lei, J. Y.; Chen, Y.; Jin, J. Protein Expr. Purif. 2012, 85, 32. doi: 10.1016/j.pep.2012.06.009
(8) Hirose, M.; Tachibana, A.; Tanabe, T. Mater. Sci. Eng. C 2010, 30, 664.(9) Li, J.; Liao, X. P.; Zhang, Q. X.; Shi, B. J. Chromatogr. B 2013, 928, 131.(10) Chen, L. H.; Zhu, G. S.; Zhang, D. L.; Zhao, H.; Guo, M. Y.; Shi,W.; Qiu, S. L. J. Mater. Chem. 2009, 19, 2013.
(11) Zhai, Z.;Wang, Y. J.; Chen, Y.; Luo, G. S. J. Sep. Sci. 2008, 31, 3527. doi: 10.1002/jssc.v31:20
(12) Wang, R.W.; Zhang, Y.; Ma, G. H.; Su, Z. G. Colloids Surf. BBiointerfaces 2006, 51, 93. doi: 10.1016/j.colsurfb.2006.05.015
(13) Marcus, R. K. J. Sep. Sci. 2008, 31, 1923.
(14) Hong, J.;Wang, Y. R.; Ye, X. H.; Zhang, Y. H. P. J. Chromatogr. B 2008, 1194, 150.
(15) Wang, S. Y.; Chen, K. M.; Kayitmazer, A. B.; Li. L.; Guo, X. H. Colloids Surf. B-Biointerfaces 2013, 107, 251. doi: 10.1016/j.colsurfb.2013.02.026
(16) Henzler, K.; Haupt, B.; Ballauff, M. Anal. Biochem. 2008, 378, 184. doi: 10.1016/j.ab.2008.04.011
(17) Wittemann, A.; Ballauff, M. Macromol. Biosci. 2005, 5, 13.
(18) Chen, K.; Zhu, Y.; Li, L.; Lu, Y.; Guo, X. Macromol. Rapid Commun. 2010, 31, 1440.
(19) Chen, K.; Zhu, Y.; Zhang, Y.; Li, L.; Lu, Y.; Guo, X. Macromolecules 2011, 44, 632. doi: 10.1021/ma102337c
(20) Ahmad, A.; Liu, X. C.; Li, L.; Guo, X. H. Adv. Chem. Eng. 2014, 44, 193. doi: 10.1016/B978-0-12-419974-3.00004-X
(21) Fang, X. L.; Gao, B. J.; Huang, X.W.; Zhang, Y. Q.; Gu, L. Y. Acta Polym. Sin. 2012, No. 12, 1472. [房晓琳, 高保娇,黄小卫, 张永奇, 顾来沅. 高分子学报, 2012, No. 12, 1472.](22) Amara, M.; Kerdjoudj, H. Talanta 2003, 60, 991. doi: 10.1016/S0039-9140(03)00155-3
(23) Yang, H.; Zheng, Q.; Cheng, R. S. Colloid Surf. A-Physicochem. Eng. Asp. 2012, 407, 1. doi: 10.1016/j.colsurfa.2012.05.031
(24) Ballauff, M.; Borisov, O. Curr. Opin. Colloid Interface Sci. 2006, 11, 316. doi: 10.1016/j.cocis.2006.12.002
(25) van der Veen, M.; Norde,W.; Stuart, M. C. Colloids Surf. BBiointerfaces 2004, 35, 33.
(26) Shamim, N.; Liang, H.; Hidajat, K.; Uddin, M. S. J. Colloid Interface Sci. 2008, 320, 15.
(27) Li,W. K.; Li, S. J. Colloid Surf. A-Physicochem. Eng. Asp. 2007, 295, 159. doi: 10.1016/j.colsurfa.2006.08.046
(28) Peng, Z. G.; Hidajat, K.; Uddin, M. S. Colloids Surf. BBiointerfaces 2004, 35, 169. doi: 10.1016/j.colsurfb.2004.03.010
(29) Bayramo?lu, G.; Ekici, G.; Be?irli, N.; Arica, M. Y. Colloid Surf. A-Physicochem. Eng. Asp. 2007, 310, 68. doi: 10.1016/j.colsurfa.2007.05.067
(30) Fu, H. Y.; Gao, B. J.; Niu, Q. Y. Acta Phys. -Chim. Sin. 2010, 26, 359. [付红艳, 高保娇, 牛庆媛. 物理化学学报, 2010, 26, 359.] doi: 10.3866/PKU.WHXB20100207
(31) Cestari, A. R.; Vieira, E. F. S.; Mattos, C. R. S. J. Chem. Thermodyn. 2006, 38, 1092. doi: 10.1016/j.jct.2005.11.011
(32) Anjos, F. S. C.; Vieira, E. F. S.; Cestaril, A. R. J. Colloid Interface Sci. 2002, 253, 243. doi: 10.1006/jcis.2002.8537
(33) Benhamou, A.; Basly, J. P.; Baudu, M.; Derriche, Z.; Hamacha, R. J. Colloid Interface Sci. 2013, 404, 135.

[1]	Mengru HAN,Yanan ZHOU,Xuan ZHOU,Wei CHU. Tunable Reactivity of MNi₁₂ (M = Fe, Co, Cu, Zn) Nanoparticles Supported on Graphitic Carbon Nitride in Methanation [J]. Acta Physico-Chimica Sinica, 2019, 35(8): 850-857.
[2]	Zhe JIANG,Fei YU,Jie MA. Design of Graphene-based Adsorbents and Its Removal of Antibiotics in Aqueous Solution [J]. Acta Phys. -Chim. Sin., 2019, 35(7): 709-724.
[3]	Wen XIE,Huan HE,Jiaxin DONG,Qinglian GUO,Yi LIU. Thermodynamics of the Interaction of Morin with Bovine Serum Albumin [J]. Acta Phys. -Chim. Sin., 2019, 35(7): 725-733.
[4]	Yan HE,Hao LI,Li ZHOU,Ting XU,Changjun PENG,Honglai LIU. Removal of Methyl Orange from Aqueous Solutions by a Novel Hyper-Cross-Linked Aromatic Triazine Porous Polymer [J]. Acta Phys. -Chim. Sin., 2019, 35(3): 299-306.
[5]	Yuxing GU,Juan YANG,Dihua WANG. Electrochemical Features of Carbon Prepared by Molten Salt Electro-Reduction of CO₂ [J]. Acta Phys. -Chim. Sin., 2019, 35(2): 208-214.
[6]	Jyotirmoy DEB,Debolina PAUL,David PEGU,Utpal SARKAR. Adsorption of Hydrazoic Acid on Pristine Graphyne Sheet: A Computational Study [J]. Acta Phys. -Chim. Sin., 2018, 34(5): 537-542.
[7]	Xuanjun WU,Lei LI,Liang PENG,Yetong WANG,Weiquan CAI. Effect of Coordinatively Unsaturated Metal Sites in Porous Aromatic Frameworks on Hydrogen Storage Capacity [J]. Acta Phys. -Chim. Sin., 2018, 34(3): 286-295.
[8]	Qiang LIU,Yong HAN,Yunjun CAO,Xiaobao LI,Wugen HUANG,Yi YU,Fan YANG,Xinhe BAO,Yimin LI,Zhi LIU. In-situ APXPS and STM Study of the Activation of H₂ on ZnO(10${\rm{\bar 1}}$0) Surface [J]. Acta Phys. -Chim. Sin., 2018, 34(12): 1366-1372.
[9]	Yuan DUAN,Mingshu CHEN,Huilin WAN. Adsorption and Activation of O₂ and CO on the Ni(111) Surface [J]. Acta Phys. -Chim. Sin., 2018, 34(12): 1358-1365.
[10]	Xinyi WANG,Lei XIE,Yuanqi DING,Xinyi YAO,Chi ZHANG,Huihui KONG,Likun WANG,Wei XU. Interactions between Bases and Metals on Au(111) under Ultrahigh Vacuum Conditions [J]. Acta Phys. -Chim. Sin., 2018, 34(12): 1321-1333.
[11]	Chen-Hui ZHANG,Xin ZHAO,Jin-Mei LEI,Yue MA,Feng-Pei DU. Wettability of Triton X-100 on Wheat (Triticum aestivum) Leaf Surfaces with Respect to Developmental Changes [J]. Acta Phys. -Chim. Sin., 2017, 33(9): 1846-1854.
[12]	Chan YAO,Guo-Yan LI,Yan-Hong XU. Carboxyl-Enriched Conjugated Microporous Polymers: Impact of Building Blocks on Porosity and Gas Adsorption [J]. Acta Phys. -Chim. Sin., 2017, 33(9): 1898-1904.
[13]	Zhou-Sheng MO,Yu-Cai QIN,Xiao-Tong ZHANG,Lin-Hai DUAN,Li-Juan SONG. Influencing Mechanism of Cyclohexene on Thiophene Adsorption over CuY Zeolites [J]. Acta Phys. -Chim. Sin., 2017, 33(6): 1236-1241.
[14]	Wei-Guo DAI,Dan-Nong HE. Selective Photoelectrochemical Oxidation of Chiral Ibuprofen Enantiomers [J]. Acta Phys. -Chim. Sin., 2017, 33(5): 960-967.
[15]	Lei HE,Xiang-Qian ZHANG,An-Hui LU. Two-Dimensional Carbon-Based Porous Materials: Synthesis and Applications [J]. Acta Phys. -Chim. Sin., 2017, 33(4): 709-728.

Adsorption Characteristics of Bovine Serum Albumin on Cationic Grafted Particles QPDMAEMA/SiO₂ with Brush Structure

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

Adsorption Characteristics of Bovine Serum Albumin on Cationic Grafted Particles QPDMAEMA/SiO2 with Brush Structure

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

Adsorption Characteristics of Bovine Serum Albumin on Cationic Grafted Particles QPDMAEMA/SiO₂ with Brush Structure