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ISSN 1000-6818CN 11-1892/O6CODEN WHXUEU
Acta Phys Chim Sin >> 2016,Vol.32>> Issue(2)>> 565-572     doi: 10.3866/PKU.WHXB201511301         中文摘要
FTIR-ATR Spectrometry of BSA Adsorption on Hydroxyapatite
YE Qing2, HU Ren1, ZHOU Jian-Zhang1, YE Yi-Wen1, XU Zhao-Xi3, LIN Chang-Jian1, LIN Zhong-Yu1
1 State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, P. R. China;
2 Chuyang Honors College, Zhejiang Normal University, Jinhua 321004, Zhejiang Province, P. R. China;
3 Xiamen AERTE System Engineering CO., LTD, Xiamen 361005, Fujian Province, P. R. China
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The microcosmic process of bovine serum albumin (BSA) adsorbing onto hydroxyapatite (HA) for different time intervals was investigated by Fourier transform infrared attenuated total internal reflectance (FTIRATR) spectrometry. The initial dissolution and re-precipitation of PO43-, Ca2+, and OH- ions from the HA coating led to the occurrence of the coating including adsorbed BSA on the HA from surface-to subsurface-molecular layers and to in-depth interaction between BSA and HA. The subtraction results gained in the adsorption regions of HA and BSA reveal that the binding of P=O, from the phosphate (PO43-), to the hydrogen of amide II, methyl and methene of the BSA appears to be considerably more rapid and stronger than that of the P―O group. In addition, it is very likely that Ca2+ plays an important role in the interaction of BSA with HA. It appears that the binding of Ca2+ to the carbonyl-oxygen of the peptide bond in BSAcaused a significant, molecular, conformational rearrangement of polypeptide backbones from β-pleated sheet to helical circles of α-helix and β-turn. This change appears to have been followed by much hydrogen of polypeptides being driven to bind PO43- and OHeffectively and much ―C=O and H―N―groups of the peptide bond being freed from inter-chain hydrogenbonding to act on Ca2+ and combine strongly with the HA surface. This might reasonably be expected to promote hard tissue regeneration. BSA seems to be activated by the inductive effect of Ca2+ via the molecular rearrangement of polypeptide backbones from pleated sheet to helical circles and in turn reacts strongly on the HA, resulting in profound effects on the course of biomineralization.



Keywords: Biomaterial   Protein adsorption   Polypeptide   IR spectroscopy   FTIR-ATR   Biomineralization  
Received: 2015-07-02 Accepted: 2015-11-26 Publication Date (Web): 2015-11-30
Corresponding Authors: LIN Zhong-Yu Email: stzylin@xmu.edu.cn

Fund: The project was supported by the National Natural Science Foundation of China (51571169).

Cite this article: YE Qing, HU Ren, ZHOU Jian-Zhang, YE Yi-Wen, XU Zhao-Xi, LIN Chang-Jian, LIN Zhong-Yu. FTIR-ATR Spectrometry of BSA Adsorption on Hydroxyapatite[J]. Acta Phys. -Chim. Sin., 2016,32 (2): 565-572.    doi: 10.3866/PKU.WHXB201511301

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56. SONG Guang-Ling;SONG Shi-Zhe.Corrosion Behaviour of Pure Magnesium in a Simulated Body Fluid[J]. Acta Phys. -Chim. Sin., 2006,22(10): 1222-1226
57. JIANG Li-Min;LI Wei-Ping;ZHU Yue-Jin .Irradiation and Recrystallization Study of Poly(vinylidene fluoride-trifluoroethylene) Copolymer By Infrared Spectrum[J]. Acta Phys. -Chim. Sin., 2006,22(08): 999-1003
58. CHEN Ming-An;XIE Xuan;QI Hai-Ying;ZHANG Xin-Ming;LI Hui-Zhong;YANG Xi .Characterization of Self-assembled Bis-[triethoxysilylpropyl]tetrasulfide Silane Films on Surface of 2A12-T6 Aluminum Alloy[J]. Acta Phys. -Chim. Sin., 2006,22(08): 1025-1029
59. CHEN Hu;XU Xing-You;GAO Jian;YANG Xu-Jie;LU Lu-De;WANG Xin.Study on Crystal Structure of [Ni(phen)3](ClO4)2[J]. Acta Phys. -Chim. Sin., 2006,22(07): 856-859
60. JIN Guo-Jie;GUO Yang-Long;LIU Xiao-Hui;YAO Wei;GUO Yun;WANG Yun-Song;YUAN Fang;LU Guan-Zhong.In situ FT-IR Study on the Direct Gas Phase Epoxidation of Propylene over Ag-MoO3 Catalyst[J]. Acta Phys. -Chim. Sin., 2006,22(07): 809-814
61. SUN Yuan-Hua;ZHANG Tong-Lai;ZHANG Jian-Guo;YANG Li;QIAO Xiao-Jing.Kinetics of Flash Pyrolysis of [Co(CHZ3](ClO42 and [Ni(CHZ)3](ClO4)2[J]. Acta Phys. -Chim. Sin., 2006,22(06): 649-652
62. WANG Xiao-Hua; TAO Guo-Hong; WU Xiao-Mu; KOU Yuan.Investigation of the Acidity of Ionic Liquids by IR Spectroscopy[J]. Acta Phys. -Chim. Sin., 2005,21(05): 528-533
63. CHEN Ren-Jie;WU Feng.Spectroscopic Investigation of New Binary Molten Salt Electrolytes Based on LiClO4 with Acetamide[J]. Acta Phys. -Chim. Sin., 2005,21(02): 177-181
64. Li Xue-Hui; Zhang Lei; Li Qiong; Geng Wei-Guo; Ye Yu-Jia; Wang Le-Fu.TGA-FTIR Study of 1-n-butyl-3-methylimidazolium Bromide Ionic Liquid[J]. Acta Phys. -Chim. Sin., 2004,20(12): 1465-1468
65. Mei Hu;Zhou Yuan;Sun Li-Li;Li Zhi-Liang.A New Descriptor of Amino Acids and Its Application in Peptide QSAR[J]. Acta Phys. -Chim. Sin., 2004,20(08): 821-825
66. Zhang Sheng-Mao;Zhang Chun-Li;Zhang Jing-Wei;Zhang Zhi-Jun;Dang Hong-Xin;Wu Zhi-Shen;Liu Wei-Min.Preparation of Silver Nanoparticles in Room Temperature Ionic Liquids[J]. Acta Phys. -Chim. Sin., 2004,20(05): 554-556
67. Li Ji-Chao;Wang Chun-Lei;Zhong Wei-Lie.Vibrational Modes Analysis of Poly (vinylidene fluoride) All-trans Molecular Chain[J]. Acta Phys. -Chim. Sin., 2003,19(11): 1010-1014
68. Yu Bin;Yang Zhan-Lan;Sun Ying;Weng Shi-Fu;Wu Jin-Guang.Interaction of Bile Salts with Calcium Phosphate[J]. Acta Phys. -Chim. Sin., 2002,18(12): 1076-1080
69. Yuan Shi-Bin;Wei Zeng-Quan;Gao Qing-Xiang;Li Hai-Yan.Radiolysis of Amino Acid Derivatives Induced by the Irradiation of 110keV Fe Ion Beams[J]. Acta Phys. -Chim. Sin., 2002,18(11): 1033-1037
70. Zhou Zhi-You;Sun Shi-Gang;Chen Sheng-Pei;Si Di;Gong Hui.Electrochemical In Situ Step-scan Time-resolved Microscope FTIR Spectroscopy[J]. Acta Phys. -Chim. Sin., 2002,18(11): 989-993
71. Sha Yin-Lin;Li Yin-Ling;Qiu Yang;Wang Qi;Lai Lu-Hua;Tang You-Qi.de novo Design and Conformational Studies of a β-hairpin Forming Peptide[J]. Acta Phys. -Chim. Sin., 2002,18(10): 907-910
72. Xu Yong-Xiang;Yan Chuan-Wei;Gao Yan-Min;Cao Chu-Nan.Diffusion and Existing Form of Water Vapour in Organic Coatings[J]. Acta Phys. -Chim. Sin., 2002,18(07): 649-652
73. Sha Yin-Lin;Huang Yong-Liang.Peptide de novo Design: CD Evidences of β-hairpin Formation by Eight-residue Peptide[J]. Acta Phys. -Chim. Sin., 2002,18(06): 504-507
74. Li Yong;Zhang Ke-Xu;Yi-Zhuang;Tao Dong-Liang;Wang Zhi-Xian;Gao Hong-Cheng;Wu Jin-Guang.Luminescence Character of Rare Earth Complexes with Derivatives of Benzoic Acid[J]. Acta Phys. -Chim. Sin., 2002,18(04): 292-296
75. Gulikezi Arip;Yang Zhan-Lan;Weng Shi-Fu;Wu Jin-Guang.The Association of Calcium Carbonate with Unconjugated Bilirubin[J]. Acta Phys. -Chim. Sin., 2001,17(10): 948-951
76. Liu Ming-Zhao;Yang Zhan-Lan;Zhang Li;Weng Shi-Fu;Wu Jin-Guang.Photoluminescence of Solid Complexes EuxM1-x(TTA)3 (H2O)2 (M=La,Gd)[J]. Acta Phys. -Chim. Sin., 2001,17(09): 797-801
77. Gao Feng;Sha Yin-Lin;Qiu Yang;Wang Yue-Feng;Li Yin-Ling;Lai Lu-Hua;Wu Hou-Ming.Solution Conformation Study on a β-turn Forming Peptide[J]. Acta Phys. -Chim. Sin., 2001,17(07): 619-621
78. Fu Hai-Tao;Li Ying;Wei Wu-Ji;Zhu Yi-Fan.The Inhibition of AMT on Bronze Corrosion in 5% Citric Acid[J]. Acta Phys. -Chim. Sin., 2001,17(07): 604-608
79. Chen Jian-Gang;Xiang Hong-Wei;Dong Qing-Nian;Wang Xiu-Zhi;Sun Yu-Han.H2 or CO Chemsorption on Co-based Catalysts for F-T Synthesis[J]. Acta Phys. -Chim. Sin., 2001,17(02): 161-164
80. Xu Gui-Ying;Zhang Li;Mao Hong-Zhi;Bao Meng;Lu Yan.The State of Solubilized Water in W/O Microemulsions in the Presence of[J]. Acta Phys. -Chim. Sin., 2001,17(01): 37-42
81. Sun Guo-Xin, Yao Ming-Ming, Cui Yu, Bao Meng, Sun Si-Xiu.Microstucture of Water Equilibrted with Organic Phase[J]. Acta Phys. -Chim. Sin., 2000,16(08): 749-752
82. Deng De-Bin, Ma Li-Jing, Liu Xiu-Ying, Li Xuan-Wen.Study of the Realumination of H Zeolite Framework by Infrared Spectroscopy[J]. Acta Phys. -Chim. Sin., 2000,16(02): 162-165
83. Chen Ming-Shu, Weng Wei-Zheng, Wan Hui-Lin.Mechanism of Propane and iso-butane Oxidehydrogenation on VMgO Catalyst[J]. Acta Phys. -Chim. Sin., 1999,15(10): 938-942
84. Feng Hai-Yan, Liu Xiao-Di, He Shu-Mei, Wu Ke-Zhong, Zhang Jian-Ling.Studies on Solid-Solid Phase Transition of Polyols and their Binary Mixtures by Infrared Spectroscopy[J]. Acta Phys. -Chim. Sin., 1999,15(09): 850-855
85. Zhang Hao-Li, Zhang Hua, Zhang Jin, Wu Bin, Liu Zhong-Fan, Li Hu-Lin.Fbrication and Structural Evaluation of Self-assembled Monolayer of Quinoline Derivative on Gold[J]. Acta Phys. -Chim. Sin., 1999,15(07): 657-661
86. Tao Peng, Wang Ren-Xiao, Lai Lu-Hua.Calculation of Peptide's Partition Coefficients by Amino Acid Addition Model[J]. Acta Phys. -Chim. Sin., 1999,15(05): 449-453
87. Li Hai-Ying, Zhang Hao-Li, Zhang Jin, Liu Zhong-Fan.Preparation and Structural Characterization of Self-assembled Monolayers from a New Series of Mercapto Contained Azobenzene Derivatives[J]. Acta Phys. -Chim. Sin., 1999,15(03): 198-203
88. Wei Kun, Shi Yan, He Lun-Yan.Crystal Structure and Infrared Spectra of Nanocrystalling Rare Earth Mixed Oxides Dy1-xSrxCoO3-y[J]. Acta Phys. -Chim. Sin., 1998,14(10): 957-960
89. Li Yan, Li Quan, Zhou Wei-Jin, Wu Jin-Guang.Spectroscopic Properties of Potassium Saponified HEHPEHE and the Formation of w/o Microemulsions[J]. Acta Phys. -Chim. Sin., 1998,14(09): 794-798
90. Mao Wen-Tao, Li Qiang, Su Hong-Mei, Kong Fan-Ao.Study of CICOCO Free Radical[J]. Acta Phys. -Chim. Sin., 1998,14(08): 673-675
91. Wang Yi-Xuan, Li Hong-Ping, Wang Jin-Ben, Zhang Fu-Qiang, Zhao Jian-Ping, Dai Ming.Infrared Spectroscopic Studies of Interactions of the Diols with Aprotic Solvents[J]. Acta Phys. -Chim. Sin., 1998,14(06): 514-519
92. Zhou Heng-Hui, Chen Ji-Tao, Ci Yun-Xiang, Liu Chang-Yan.A Simple Method to Evaluate Lithium Insertion Capacity of PPP-based Carbon[J]. Acta Phys. -Chim. Sin., 1998,14(05): 477-480
93. Ding Han-Ming, Wang Hai-Shui, Cui Hai-Ning, Xi Shi-Quan.Study of Molecular Orientation in Langmuir-Blodgett Films by FTIR[J]. Acta Phys. -Chim. Sin., 1998,14(05): 472-476
94. Wang Bao-Shan, Guo Jing-Zhong, Gu Yue-Shu, Mao Wen-Tao, Kong Fan-Ao.Vibrational Energy Transfer from Highly Excited State CO to C2H2[J]. Acta Phys. -Chim. Sin., 1998,14(04): 327-331
95. Zhang Jin, Zhao Jiang, He Hui-Xin, Zhang Hao-Li, Li Hu-Lin, Liu Zhong-Fan.Mutual Coupling of Surface Electromagnetic Wave Between Silver Particles: Studies on the Mechanism of the Surface Enhanced Infrared Spectroscopy[J]. Acta Phys. -Chim. Sin., 1998,14(04): 375-379
96. Liu Liang,Wang Ren-Xiao,Lai Lu-Hua,Li Chong-Xi.3D-QSAR and Pharmacophore Modeling of Growth Hormone Secretagogues[J]. Acta Phys. -Chim. Sin., 1997,13(12): 1090-1096
97. Li Qiang,Mao Wen-Tao,Li Hong-Zhi,Zhu Qi-He,Kong Fan-Ao,Huang Ming-Bao.A Study of Excited States of Acetyl Radical[J]. Acta Phys. -Chim. Sin., 1997,13(10): 879-884
98. Zhang Jin,Zhao Jiang,Zhang Hao-Li,Liu Zhong-Fan,Li Hu-Lin.Influence of End Alkyl Chain Length on the Structure of Self-assembled Monolayer of Azobenzene on Gold[J]. Acta Phys. -Chim. Sin., 1997,13(10): 868-872
99. Zhang Hao-Li,Zhang Jin,Zhao Jiang,Wang Yong-Qiang,She Jin,Yu Hua-Zhong,Li Hu-Lin.New Approach on the Synthesis of Functionalized Alkanethiols and the Structural Characterization of Their Self-Assembled Monolayers[J]. Acta Phys. -Chim. Sin., 1997,13(06): 515-524
100. Li Quan,Li Wei-Hong,Weng Shi-Fu,Wu Jin-Guang,Xu Guang-Xian.FT-IR Study of Water Structure in Water/AOT/n-Heptane Microemulsion System[J]. Acta Phys. -Chim. Sin., 1997,13(05): 438-444
101. Ke Jie,Jin Shun-Zi,Han Bu-Xing,Yan Hai-Ke,Shen De-Yan.FTIR Study on the Interactions between Benzoic Acid and Ethanol,Benzoic Acid and Dimethyl Sulfoxide in Supercritical CO2[J]. Acta Phys. -Chim. Sin., 1996,12(11): 986-989
102. Ding Xiao-Qin,Chen Chang-Ying,Li Yu-Lin,Chen Ji-Sheng,Luo Yu,Lai Lu-Hua,Xu Xiao-.Quantitative Structure-activity Relationships for μ-conotoxin GIIIA and its Derivatives[J]. Acta Phys. -Chim. Sin., 1996,12(06): 485-490
103. Yang Hui,Lu Tian-Hong,Xue Kuan-Hong,Zhou Yi-Ming,Sun Shi-Gang,Chen Sheng-Pei.Cyclic Voltammetry and in-situ FTIR Spectroscopic Studies on Electro-oxidation of Formaldehyde at a Gold Electrode[J]. Acta Phys. -Chim. Sin., 1996,12(06): 527-531
104. Huang Wen,Li Xiao-Feng,Gu Ti-Ren.Conformation and Activity of RNaseA in Dodecylammonium Butyrate-Cyclohexane Reverse Micellar Solution[J]. Acta Phys. -Chim. Sin., 1996,12(04): 353-356
105. Wang Bi-Xun,Fu Yi-Lu,Fang Shu-Nong.A Study of [Cu-O-Cu]2+ Species on Cu-ZSM-5 Zeolite by in situ Infrared Spectroscopy[J]. Acta Phys. -Chim. Sin., 1995,11(11): 974-978
106. Yu Lin,Xu Yi-De,Guo Xie-Xian.A FT-IR Study on the Interaction of CH4 and O2 with the Surface of SrO-La2O3/CaO Catalysts[J]. Acta Phys. -Chim. Sin., 1995,11(10): 902-906
107. Li Ya-Li, Gao Yang, Liang Yong, Zheng Feng, Xiao Ke-Shen, Hu Zhuang-qi.Study of Surfacial Structure of Nano Si3N4 by Fourier Infrared Diffusion Reflection Spectra[J]. Acta Phys. -Chim. Sin., 1995,11(10): 886-889
108. Li Run-Qing, Zhang Jie, Chen Hui-Ming, Li Yu-He, Xu Xiao-Hua, Xie Qing-Lan.Study on the Mechanism of the Solvent Effect of Infrared Spectra(IV)[J]. Acta Phys. -Chim. Sin., 1995,11(09): 844-850
109. Zhang Fu-Shun,Wu Zhi-Yun,Kan Qiu-Bin.Study on IR Spectra of Ti-ZSM-11 Zeolite[J]. Acta Phys. -Chim. Sin., 1995,11(09): 836-839
110. Hu Yong-Ren,Zhang Lan-Xin,Xin Qin,Gao Xing-Tao,Ying Pin-Liang.Infrared Studies of the Surface Structure of Commercial Pt-Re/Al2O3 Reforming Catalyst[J]. Acta Phys. -Chim. Sin., 1995,11(07): 636-641
111. Li Jun, Li Xin-Sheng, Zhou Jian-Lue, Xin Qin, Zhang Hui, Chen Yao-Qiang, Chen Yu.Co or/and NO Adsorption on Reduced Rh2/SiO2 and Rh2-V/SiO2 Catalysts Characterized by Infrared Spectroscopy[J]. Acta Phys. -Chim. Sin., 1995,11(05): 401-406
112. Zhou Wei-Jin, Shi Nai, Wu Jin-Guang.The Formation of Microemulsion Systems of Alkali Metal Salts of a 2-Ethylhexyl Phosphonic Acid Mono 2-Ethylhecyl Ester and the FT-IR Spectroscopic Studies[J]. Acta Phys. -Chim. Sin., 1995,11(02): 162-166
113. Sun Shi-Gang, Lu Guo-Qiang.Kinetics and Time Resolved FTIR Spectroscopic Characters of Dissociative Adsorption of HCOOH on Pt Electrode[J]. Acta Phys. -Chim. Sin., 1995,11(01): 56-60
114. Li Xin-Sheng, Xin Qin, Zhang Hui, Li Jun, Zhou Jian-Lue, Chen Yao-Qiang, Chen Yu.Characterization of CO and/or NO Adsorbed on Reduced Rh-V/SiO2 Catalyst by Infraed Spectroscopy[J]. Acta Phys. -Chim. Sin., 1994,10(11): 1009-1014
115. Lai Lu-Hua, Wang Le-Yu, Deng Qiao-Lin, Han Yu-Zhen, Ma Li-Bin, Xu Xiao-Jie, Tang You-Qi.Constraint Monte-Carlo Simulated Annealing:Application in Peptide Conformational Analysis in Solution[J]. Acta Phys. -Chim. Sin., 1994,10(10): 867-869
116. Zheng Yu, Xie Wei-Wen, Zhang Lin-Na, Fu Gui-Xiang, Yu Lu, Zhu Zi-Ying.Thermal Decompositon Process and the Fragmentation Regularity of FAB-Mass Spectra of the Complex [Na3Fe(C2O4)3]·5H2O[J]. Acta Phys. -Chim. Sin., 1994,10(09): 774-778
117. Tian Zhong-Qun, Sun Shi-Gang, Luo Jin, Yang Yong.[J]. Acta Phys. -Chim. Sin., 1994,10(09): 860-866
118. Li Jing-Jian, Diao Peng, Cai Sheng-Min, Hou Yong-Tian, Wang Xin, Zhang Shu-Lin.The Influence of Chmical Oxidation on Surface State and Photoluminescence of Porous Silicon[J]. Acta Phys. -Chim. Sin., 1994,10(08): 737-740
119. She Li-Qin, Zhu Gao-Zhong, Wen Rui-Wu, Zhang Chang-Jun, Li Xuan-Wen, Liu Xing-Yun.Formation and Characterization of Ga Active Site in GaZSM-5 Zeolite[J]. Acta Phys. -Chim. Sin., 1994,10(07): 628-634
120. Wang Yi-Xuan, Li Hong-Ping, Zhang Fu-Qiang, Zhao Jian-Ping, Dai Ming.A Spectral Study of Intermolecular Hydrogen Bonding for N-methylaniline or Diphenylamine with some Proton Acceptors[J]. Acta Phys. -Chim. Sin., 1994,10(03): 217-222
121. She Li-Qin, Wang Duo-Cai, Li Xuan-Wen, Liu Xing-Yun, Han Ming.The States of Zinc in ZnZSM-5 Zeolites and their Catalysis[J]. Acta Phys. -Chim. Sin., 1994,10(03): 247-253
122. Lai Lu-Hua; Xu Hui-Juan; Wang Peng-Liang; Xu Xiao-Jie; Miao Zhen-Wei; Ji Ai-Xue.A Simplified Systematic Search Method and its Application in Peptide Conformational Analysis[J]. Acta Phys. -Chim. Sin., 1993,9(05): 581-583
123. Zeng Guang-Fu; Guo Xin; Wang Cui-Ying; Lin Yong-Hua; Li Han.Infrared Spectra and Crystal Structure of BIS-(Dimethoxylphosphato) Copper[J]. Acta Phys. -Chim. Sin., 1992,8(06): 778-782
124. Guo Ning; Zeng Guang-Fu; Xi Shi-Quan.Thermal Analysis nad Infrared Spectra of Dialkylammonium Tetrachlorocuprate[J]. Acta Phys. -Chim. Sin., 1992,8(06): 783-788
125. Zhou Zhi-Hua; Hu Wei-Dong; Lu Wen-Qing; Zhou Yi-Ming; Xue Kuan-Hong.Effect of Supporting Electrolyte on the Electrochemical Oxidation Pathways of Anhydrous Methanol at Gold Electrode[J]. Acta Phys. -Chim. Sin., 1992,8(05): 707-711
126. Wang Jin-Ben; Wang Yi-Xuan; Zhang Fu-Qiang; Zhao Jian-Ping; Dai Ming.A Study on Association of Amine With Several Solutes by Infrared Spectrometry[J]. Acta Phys. -Chim. Sin., 1992,8(04): 481-486
127. Xiao Feng-Shou; Ying Pin-Liang; Xin Qin; Guo Xie-Xian.A Characterization of Various Co-SITES on Co-Mo/Al2O3 and Ru-Co-Mo/Al2O3 Catalysts[J]. Acta Phys. -Chim. Sin., 1992,8(03): 321-325
128. Xu Jian-He; Sun Xian-Da; Lu Wei-Qin; Gao Hong-Jin.Study of Hydrogen Bond Association of Alcohol Molecules in Alkanes by IR Quantitative Analysis[J]. Acta Phys. -Chim. Sin., 1992,8(03): 358-363
129. Li Xin-Sheng; Hou Zhen-Shan; Wei Zhao-Bin; Xin Qin.Study on Promotion of Cobalt and Ruthenium in Reduced Catalysts for Hydrodesulfurization by IR-Spectroscopy and Chemisorption[J]. Acta Phys. -Chim. Sin., 1991,7(06): 673-680
130. Wang Yi-Xuan; Zhao Jian-Ping; Dai Ming.Infrared Spectra Studies of Butanols in Carbon Tetrachloride, n-Heptane, Benzene and 1,2-Dichloroethane[J]. Acta Phys. -Chim. Sin., 1991,7(01): 36-42
131. Liang Ying-Qiu; Jiang Yu-Tao; Tian Yong-Chi.FT-IR Spectroscopic Study on Langmuir-Blodgett Films of Stearic Acid[J]. Acta Phys. -Chim. Sin., 1991,7(01): 72-76
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