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Acta Physico-Chimica Sinca  2016, Vol. 32 Issue (12): 2951-2960    DOI: 10.3866/PKU.WHXB201609231
ARTICLE     
The Interactions between Quaternary Ammonium Cationic Surfactants and Bovine Serum Albumin
Hu-Jun XIE1,*(),Cheng-Cheng LIU1,Qiang SUN1,Qing GU2,*(),Qun-Fang LEI3,Wen-Jun FANG3,*()
1 Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310018, P. R. China
2 School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P. R. China
3 Department of Chemistry, Zhejiang University, Hangzhou 310028, P. R. China
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Abstract  

UV-visible (UV-Vis) absorption spectroscopy, fluorescence spectroscopy (FL), dynamic light scattering (DLS) and isothermal titration calorimetry (ITC) were used to study the interactions between bovine serum albumin (BSA) and the three quaternary ammonium surfactants N-dodecyl-N-(2-hydroxyethyl)-N, Ndimethyl ammonium bromide (DHDAB), N-tetradecyl-N-(2-hydroxyethyl)-N, N-dimethyl ammonium bromide (THDAB) and N-cetyl-N-(2-hydroxyethyl)-N, N-dimethyl ammonium bromide (CHDAB). These surfactants quenched the intrinsic fluorescence of BSA, with longer alkyl chains resulting in more significant quenching. This was attributed to static quenching. Further evidence of static quenching was provided by UV-Vis absorption spectroscopy. The particle size of BSA was found to initially increase and then decrease with increasing surfactant concentration. The concentration of surfactant changed the type of interaction mode. This work revealed the mechanism and binding characteristics between surfactants and protein, and provides the basis for further applications of surfactants.



Key wordsSurfactants      Bovine serum albumin      Fluorescence quenching      Dynamic light scattering      Isothermal titration calorimetry     
Received: 18 July 2016      Published: 23 September 2016
MSC2000:  O648  
Fund:  The project was supported by the National Natural Science Foundation of China(21203166,21473157);Natural Science Foundation of ZhejiangProvince, China(LY16B030001);and Food Science and Engineering the Most Important Discipline of Zhejiang Province, China(JYTsp2014111)
Corresponding Authors: Hu-Jun XIE,Qing GU,Wen-Jun FANG     E-mail: hujunxie@gmail.com;guqing2002@hotamail.com;fwjun@zju.edu.cn
Cite this article:

Hu-Jun XIE,Cheng-Cheng LIU,Qiang SUN,Qing GU,Qun-Fang LEI,Wen-Jun FANG. The Interactions between Quaternary Ammonium Cationic Surfactants and Bovine Serum Albumin. Acta Physico-Chimica Sinca, 2016, 32(12): 2951-2960.

URL:

http://www.whxb.pku.edu.cn/10.3866/PKU.WHXB201609231     OR     http://www.whxb.pku.edu.cn/Y2016/V32/I12/2951

 
 
 
Surfactant T/K Stern-Volmer Lineweaver-Burk
Ra Ksv/(L?mol-1) Kq/(L?mol-1?s-1) Ra KA/(L?mol-1) n
DHDAB 289 0.999 1.16×103 1.16×1011 0.999 1.68×103 1.039
298 0.998 0.71×1〇3 0.71×1011 0.997 1.23 x103 1.056
THDAB 289 0.997 4.08×103 4.08×1011 0.999 2.19×103 0.936
298 0.997 2.93×103 2.93×1011 0.999 1.71 x103 0.944
CHDAB 289 0.995 8.48×103 8.48×1011 0.993 8.03×103 0.993
298 0.995 7.53×103 7.53×1011 0.997 4.26×103 0.939
 
 
 
 
 
 
 
 
 
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