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Acta Physico-Chimica Sinca  2017, Vol. 33 Issue (5): 1001-1009    DOI: 10.3866/PKU.WHXB201701131
Benzo[a]pyrene Sensing Properties of Surface Plasmon Resonance Imaging Sensor Based on the Hue Algorithm
Zhi-Bo FAN1,3,Xiao-Qing GONG1,3,Dan-Feng LU1,Ran GAO1,Zhi-Mei QI1,2,*()
1 State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China
2 State Key Laboratory of NBC Protection for Civilian, Beijing 102205, P. R. China
3 University of Chinese Academy of Sciences, Beijing 100049, P. R. China
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A colorful surface plasmon resonance imaging sensor for the in-situ detection of benzopyrene (BaP) in water is presented in this paper. The sensor can provide intuitive image information and can also quantitatively analyze the concentration and adsorption/desorption processes of the analyte by combining the hue algorithm. Both the resonance wavelengths and resonance images for a bare gold film chip were obtained at different incident angles using a home-made surface plasmon resonance (SPR) sensor that possesses wavelength-interrogating and imaging capabilities. The relationship between the resonance wavelength and the average hue of the color image was established based on the hue algorithm. From this relationship, the initial resonance wavelength at which the SPR sensor can provide optimal hue sensitivity was derived, which was ~650 nm. Polytetrafluoroethylene (PTFE)-coated SPR sensor chips were prepared for the in-situ rapid detection of BaP in water based on the reversible enrichment of BaP molecules in the PTFE film. The results showed that: (1) the average hue of the SPR color image decreases linearly as BaP concentration increases from 20 to 100 nmol·L-1; (2) both the response time and recovery times of the SPR sensor for 100 nmol·L-1 BaP are 7 and 5 s, respectively; (3) since the thickness of the PTFE filmis greater than the penetration depth of the surface plasmon field, the BaP detection is not affected by the refractive index of the solution sample; and (4) in the case of a non-uniform PTFE film, the sensor allows to determine the hue sensitivities for equal-thickness microscale areas of the sensing film. The experimental results show that this type of colorful SPR imaging sensor has widespread applicability for chemical and biological detection.

Key wordsColor surface plasmon resonance imaging      Hue      Benzo[a]pyrene      PTFE sensing film      In-situ detection     
Received: 01 December 2016      Published: 13 January 2017
MSC2000:  O647  
Fund:  the National Key Basic Research Program of China (973)(2015CB352100);National Natural Science Foundation of China(61377064);National Natural Science Foundation of China(61675203);and Research Equipment Development Project of Chinese Academy of Sciences(YZ201508)
Corresponding Authors: Zhi-Mei QI     E-mail:
Cite this article:

Zhi-Bo FAN,Xiao-Qing GONG,Dan-Feng LU,Ran GAO,Zhi-Mei QI. Benzo[a]pyrene Sensing Properties of Surface Plasmon Resonance Imaging Sensor Based on the Hue Algorithm. Acta Physico-Chimica Sinca, 2017, 33(5): 1001-1009.

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Fig 1 Schematic diagram of experimental setup CCD: charge-coupled device; SPR: surface plasmon resonance
Fig 2 (a) Resonance spectra measured at different incident angles; (b) SPR images corresponding to different resonance spectra; (c) two-dimensional (2D) hue profiles corresponding to different SPR images; (d) average hue versus resonance wavelength
Fig 3 (a) SPR images and the corresponding 2D hue profiles measured in air and water; (b) resonance spectra measured in air and water claddings
Fig 4 (a) SPR images recorded with aqueous BaP solutions of different concentrations and the corresponding 2D hue profiles; (b) average hue versus BaP concentration
Fig 5 (a) SPR images recorded at different adsorption time and the corresponding 2D hue profiles; (b) average hue versus adsorption time
Fig 6 (a) SPR images recorded at different desorption time and the corresponding 2D hue profiles; (b) average hue versus desorption time
Fig 7 (a) and (b) SPR images and the corresponding 2D hue profiles measured before and after adsorption insets: magnified view of the selected regions
Fig 8 (a) Resonance spectra measured before and after adsorption; (b) comparison of the hue changes for two selected regions (ΔλR for the area of spot was also shown)
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