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Acta Physico-Chimica Sinca  2017, Vol. 33 Issue (5): 1051-1056    DOI: 10.3866/PKU.WHXB201702201
ARTICLE     
Reactions of Triplet-State Difloxacin with Amino Acids and dGMP: A Laser Flash Photolysis Study
Hai-Xia LI1,Yan-Cheng LIU2,Rui-Zhi TANG2,Peng ZHANG2,Liu-Kui MA1,Chi-Yuan WEI1,Wen-Feng WANG2,*()
1 Pujiang Institute, Nanjing Tech University, Nanjing 211132, P. R. China
2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China
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Abstract  

The reactions of triplet-state difloxacin (DFX) with various amino acids and deoxyguanylic acid in aqueous media were studied using laser flash photolysis. Tryptophan, tyrosine, cysteine, and 2'-deoxyguanosine-5'-monophosphate (dGMP) were found to completely quench the triplet state of DFX in aqueous solution, the corresponding second-order rate constants being 1.97×108, 1.48×108, 1.72×108, and 6.92×107 dm3·mol-1·s-1. The quenching mechanism involves electron transfer to the photoexcited triplet state of DFX from the tryptophan, tyrosine, cysteine, and dGMP moieties, followed by fast protonation of the resulting DFX anion radical.



Key wordsLaser flash photolysis      Difloxacin      Triplet state      Amino acid     
Received: 18 November 2016      Published: 20 February 2017
MSC2000:  O644  
Fund:  The project was supported by the National Natural Science Foundation of China(21173252)
Corresponding Authors: Wen-Feng WANG     E-mail: wangwen@sinap.ac.cn
Cite this article:

Hai-Xia LI,Yan-Cheng LIU,Rui-Zhi TANG,Peng ZHANG,Liu-Kui MA,Chi-Yuan WEI,Wen-Feng WANG. Reactions of Triplet-State Difloxacin with Amino Acids and dGMP: A Laser Flash Photolysis Study. Acta Physico-Chimica Sinca, 2017, 33(5): 1051-1056.

URL:

http://www.whxb.pku.edu.cn/10.3866/PKU.WHXB201702201     OR     http://www.whxb.pku.edu.cn/Y2017/V33/I5/1051

 
 
 
 
 
Amino acid pH Abbreviated formula
tryptophan 7.4 TrpH
tyrosine 7 TyrOH
11 TyrO-
cysteine 6.5 CysH
10.5 Cys-
 
 
 
 
Compound TrpH TyrOH CysH dGMP
DFX 1.97 × 108 3.14 × 108 - 6.92 × 107
 
 
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