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ISSN 1000-6818CN 11-1892/O6CODEN WHXUEU
Acta Phys Chim Sin >> 2017,Vol.33>> Issue(10)>> 2052-2057     doi: 10.3866/PKU.WHXB201750105         中文摘要
Study on the Synthesis of DNA via Rolling Circle Amplification
LIU Shu-Zhen, ZHANG Zhi-Qing, WANG Fang, ZHOU Ting, WANG Xiu-Feng, ZHANG Gou-Dong, LIU Ting-Ting, ZHANG Hong-Zhi
School of Science, China University of Petroleum(East China), Qingdao 266580, Shandong Province, P. R. China
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Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic reaction, which has developed as a novel technology in the field of nucleic acid amplification. Its product has a wide range of applications in the assembly and preparation of multi-functional materials. Here, we report the effects of reaction time and the concentrations of deoxyribonucleoside triphosphates (dNTPs), polymerase, and primer on the product of RCA. The RCA product was characterized by methods including agarose gel electrophoresis, ultraviolet spectroscopy, and transmission electron microscopy (TEM). The results showed that the length of the RCA product was significantly affected by the reaction time, especially when the reaction time was less than 30 min. With an increase of dNTPs concentrations, the concentration and chain length of the RCA product increased. However, while the concentrations of enzyme and primer had little effect on the length of the RCA product, they had a large effect on its concentration. It is worth noting that the content of the RCA product decreased significantly in the presence of excess enzyme concentration.



Keywords: Rolling circle amplification   Nucleic acid   dNTPs   Polymerase   Primer  
Received: 2017-03-29 Accepted: 2017-05-02 Publication Date (Web): 2017-05-10
Corresponding Authors: ZHANG Zhi-Qing Email: zhangzq@upc.edu.cn

Fund: The project was supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars (2014010615), National Natural Science Foundation of China (21603276, 21303267), Natural Science Foundation of Shandong Province, China (ZR2016BL14), and Fundamental Research Funds for the Central Universities, China.

Cite this article: LIU Shu-Zhen, ZHANG Zhi-Qing, WANG Fang, ZHOU Ting, WANG Xiu-Feng, ZHANG Gou-Dong, LIU Ting-Ting, ZHANG Hong-Zhi. Study on the Synthesis of DNA via Rolling Circle Amplification[J]. Acta Phys. -Chim. Sin., 2017,33 (10): 2052-2057.    doi: 10.3866/PKU.WHXB201750105

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