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ISSN 1000-6818CN 11-1892/O6CODEN WHXUEU
Acta Phys Chim Sin >> 0,Vol.>> Issue()>> 0-0     doi: 10.3866/PKU.WHXB201707043         中文摘要
Accepted manuscript
Microcalorimetric Analysis of Isolated Rat Liver Mitochondrial Metabolism under Different Conditions
YUAN Lian1, LIU Yu-Jiao1, HE Huan1, JIANG Feng-Lei1, LI Hui-Rong2, LIU Yi1
1 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China;
2 College of Life Science and Chemistry, Wuhan Donghu University, Wuhan 430212, P. R. China
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Isolated rat liver mitochondria were proposed as a model to monitor real-time heat metabolism.A high-throughput and sensitive thermal activity monitor III (TAM III) was used to detect the P-t curves of mitochondria under different conditions,including different mitochondrial concentrations,different substrates,different buffers,respiratory inhibitors,Ca2+,and CsA.We determined the thermokinetic parameters through calculation.The results showed that:(1) higher concentration of mitochondria led to faster energetic metabolism;(2) when succinate was the direct respiratory substrate,it promoted mitochondrial metabolism,in contrast to the condition when an indirect substrate,pyruvate,was used;(3) high concentration of Ca2+(2.5 mmol·L-1) stimulated mitochondrial metabolism,however CsA,an inhibitor of mitochondrial permeability transition pores,could not reverse the Ca2+-induced mitochondrial alteration;(4) mitochondria in various buffers displayed different rates of heat metabolism,because of the different composition of the buffers;(5) mitochondrial metabolism was inhibited by respiratory inhibitors,especially NaN3,which is an inhibitor of Complex IV and which completely stopped the mitochondrial heat release.



Keywords: Mitochondria   Microcalorimetry   Metabolic rate   Respiratory inhibitor   Mitochondrial permeability transition  
Received: 2017-06-13 Accepted: 2017-06-19 Publication Date (Web): 2017-07-04
Corresponding Authors: LIU Yi Email: yiliuchem@whu.edu.cn

Fund: The project was supported by the National Natural Science Foundation of China (21673166) and Natural Science Foundation of Hubei Province,China (2015CFC892).

Cite this article: YUAN Lian, LIU Yu-Jiao, HE Huan, JIANG Feng-Lei, LI Hui-Rong, LIU Yi. Microcalorimetric Analysis of Isolated Rat Liver Mitochondrial Metabolism under Different Conditions[J]. Acta Phys. -Chim. Sin., 0, (): 0-0.    doi: 10.3866/PKU.WHXB201707043

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