物理化学学报 >> 1995, Vol. 11 >> Issue (10): 957-960.doi: 10.3866/PKU.WHXB19951022

研究简报 上一篇    

水溶性金属卟啉与DNA相互作用的微量热法研究

熊亚,黄素秋,吴鼎泉,屈松生   

  1. 武汉大学化学系，武汉 430072
  • 收稿日期:1994-12-26 修回日期:1995-03-25 发布日期:1995-10-15
  • 通讯作者: 屈松生

The Interaction of Water-soluble Quaternary Ammonium Pridine Pophyrins with DNA Studied by Microcalorimetry

Xiong Ya,Huang Su-Qiu,Wu Ding-Quan,Qu Song-Sheng   

  1. Department of Chemistry,Wuhan University,Wuhan 430072
  • Received:1994-12-26 Revised:1995-03-25 Published:1995-10-15
  • Contact: Qu Song-Sheng

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关键词: 水溶性金属卟啉, DNA, 微量热法

Abstract:

The interactions of water-soluble metal porphyrins Cu(NECN) and Ni(NEAE) with calf thymus ds DNA and ss DNA have been investigated by microcalarimetry and UV spectroscopy. The results demonstrat that the reaction of Cu(NACN) with ds DNA is endothermic with the binding enthalpy △H=9.2 kJ•mol-1 for Cu(NACN), its saturated binding number is 4~5 base pairs, the reaction of Ni(NEAE) with ds DNA is exothermal with the binding enthalpy △H=-7.6 kJ•mol-1 for Ni(NEAE), its saturated binding number is 5-6 base pairs, the difference of their binding modes are mainly attributed to their metal ionic properties in porphyrine molecules which are studied in this paper. Watersoluble porphyrins, Cu(NACN) and Ni(NEAE) interact with ss DNA more intensive.

Key words: Watr-soluble metal porphyrin, DNA, Microcalorimetry