Acta Phys. -Chim. Sin. ›› 1997, Vol. 13 ›› Issue (01): 71-73.doi: 10.3866/PKU.WHXB19970115

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Microcalorimetric Study on the Respiratory Burst of Polymorphonuclear Leukocytes

Tan An-Min,Huang Yuan-Qing,Qu Song-Sheng   

  1. Department of Chemistry,Wuhan University,Wuhan 430072|National Key Laboratory of Natural and Biomimetic Drugs,Beijing 100083|Ningxia Medical University,Yingchuan 650004
  • Received:1996-07-16 Revised:1996-09-25 Published:1997-01-15
  • Contact: Qu Song-Sheng

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Abstract:

The heat production of Wistar rat polymorphonuclear leukocytes (PMN) was measured by an LKB 2277 Thermal Activity Monitor. When PMN were activited with phorbol-12-myristate13-acetate (PMA), the respiratory burst was recorded by greatly incr eased heat production. Experiment was also carred out in the present of the inhibitor, Total Flavonoids of Lycium Barbarum L. (TFL). The respiratory burst heat production peak was disappeared, but the heat production curve was higher than that of PMA because TFL increased the metabolic activities of PMN.

Key words: Thermochemistry, Microcalorimetry, Respiratory burst, Polymorphonuclear leukocytes(PMN), Phorbol-12-myristate-13-acetate(PMA), Total Flavonoids of Lycium barbarum L.(TFL)