微量热法研究环境NaCl浓度对盐生盐杆菌生长代谢的影响

doi:10.3866/PKU.WHXB20110618

Acta Phys. -Chim. Sin. ›› 2011, Vol. 27 ›› Issue (06): 1525-1530.doi: 10.3866/PKU.WHXB20110618

• BIOPHYSICAL CHEMISTRY • Previous Articles Next Articles

Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu

ZENG Chi¹, ZHU Jian-Yu²

1. School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China;
2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, P. R. China

Received:2011-02-16 Revised:2011-04-05 Published:2011-05-31
Contact: ZENG Chi, ZHU Jian-Yu E-mail:zeng_chi@yahoo.com.cn; csuzhu@yahoo.com.cn
Supported by:
The project was supported by the National Natural Science Foundation of China (31000050, 30700008) and Research Program of Hubei Provincial Department of Education, China (B20111701).

Abstract

Abstract:

Microcalorimetry was used to study the influence of extracellular NaCl concentration on the growth metabolism of Halobacterium halobium (Hbt. halobium) using a TAM air isothermal microcalorimeter. The metabolic thermogenic curves of Hbt. halobium growth in different concentrations of NaCl were obtained using the ampoule method. The thermokinetic equations and parameters of Hbt. halobium growth were calculated and the relationship between each thermokinetic parameter and the concentration of NaCl was obtained. The results showed that the optimum NaCl concentration for Hbt. halobium growth was not a wide range from 3.5 mol L^-1 to about 5.2 mol·L^-1 (NaCl saturation), as is generally acknowledged, but just around 3.9 mol·L^-1. For an extracellular NaCl concentration above 3.9 mol·L^-1, the growth metabolism of Hbt. halobium decreased constantly with an increase in the NaCl concentration. Further investigation by transmission electron microscopy revealed that the Hbt. halobium cells growing in approaching NaCl saturation underwent plasmolysis, which interpreted the finding of microcalorimetry perfectly. All these results led to a new interpretation of the structural transformations of Hbt. halobium upon NaCl concentration altering.

Key words: Microcalorimetry, Haloarchaea, Extracellular NaCl concentration, Cell surface structure

ZENG Chi, ZHU Jian-Yu. Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu[J]. Acta Phys. -Chim. Sin. 2011, 27(06), 1525-1530. doi: 10.3866/PKU.WHXB20110618

References

(1) Oren, A. The Order Halobacteriales. In The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 3rd ed.; Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H., Stackebrandt, E. Eds.; Springer-Verlag: New York, 1999.
(2) Grant,W. D.; Kamekura, M.; McGenity, T. J.; Ventosa, A. Halobacteria. In Bergey’ s Manual of Systematic Bacteriology, 2nd ed.; Garrity, G. M. Eds.; Springer-Verlag: New York, 2001.
(3) Stoeckenius,W.; Rowen, R. J. Cell Biol. 1967, 34, 365.
(4) Grant,W. D. Phil. Trans. R. Soc. Lond. B 2004, 359, 1249.
(5) Willey, J.; Sherwood, L.;Woolverton, C. Prescott′s Microbiology, 8th ed.; McGraw-Hill: New York, 2011; chap. 6.
(6) Zeng, C.; Zhu, J. C.; Liu, Y.; Yang, Y.; Zhu, J. Y.; Huang, Y. P.; Shen, P. J. Therm. Anal. Cal. 2006, 84, 625.
(7) Wads?, I. Thermochim. Acta 2002, 394, 305.
(8) Braissant, O.;Wirz, D.; G?pfert, B.; Daniels, A. U. FEMS Microbiol. Lett. 2010, 303, 1.
(9) Rodriguez-Valera, F.; Ruiz-Berraquero, F.; Ramos-Cormenzana, A. J. Gen. Microbiol. 1980, 119, 535.
(10) Gibbons, N. E.; Payne, J. I. Can. J. Microbiol. 1961, 7, 483.
(11) Pritchard, J. Turgor Pressure. In Encyclopedia of Life Sciences; Nature Publishing Group: London, 2001.
(12) Koch, A. L. Growth Measurement. In Manual of Methods for General Bacteriology; Gerhardt, P. Eds.; American Society for Microbiology:Washington D.C., 1981; chap. 11.
(13) Shand, R. F.; Perez, A. M. Haloarchaeal Growth Physiology. In Enigmatic Organisms and Life in Extreme Environments; Seckback, J. Eds.; Kluwer Academic Publishers: Dordrecht, 1999; pp 413-424.
(14) Dyall-Smith, M. The Halohandbook: Protocols for Haloarchaeal Genetics, version 7.1; 2009; pp 109-111. http://www.haloarchaea.com/resources/halohandbook
(15) Huang, Y. P.; Liu, Y.; Shen, P.; Qu, S. S. J. Therm. Anal. Cal. 2003, 74, 163.
(16) Liu, P.; Liu, Y.; Xie, Z. X.; Chen, Y. G.; Zhao, R. M.; Shen, P.; Qu, S. S. Chin. J. Chem. 2003, 21, 693.
[刘鹏, 刘义, 谢之雄, 陈酉贵, 赵儒铭, 沈萍, 屈松生. 中国化学, 2003, 21, 693.]
(17) Liu, Y.; Yu, H. G.; Huang, Y. P.; Shen, P.; Qu, S. S. Biol. Trace Elem. Res. 2003, 92, 83.

[1]	Wen Xie,Lianjiao Zhou,Juan Xu,Qinglian Guo,Fenglei Jiang,Yi Liu. Advances in Biothermochemistry and Thermokinetics [J]. Acta Physico-Chimica Sinica, 2020, 36(6): 1905051-.
[2]	Lian YUAN,Yu-Jiao LIU,Huan HE,Feng-Lei JIANG,Hui-Rong LI,Yi LIU. Microcalorimetric Analysis of Isolated Rat Liver Mitochondrial Metabolism under Different Conditions [J]. Acta Phys. -Chim. Sin., 2018, 34(1): 73-80.
[3]	Shun-Sheng ZHAO,Lan-Lan LI,Xiang-Rong LIU,Zuo-Cheng DING,Zai-Wen YANG. Crystal Structure, Thermal Decomposition and Interaction with CT-DNA of Three 2-Hydroxy-1-naphthaldehyde Acyl Hydrazones [J]. Acta Phys. -Chim. Sin., 2017, 33(2): 356-363.
[4]	Huan-Feng TANG,Zai-Yin HUANG,Ming XIAO,Min LIANG,Li-Ying CHEN. An Investigation into the Reaction Kinetics of Cubic Nano-Cu₂O in Theory and Experiment [J]. Acta Phys. -Chim. Sin., 2016, 32(12): 2891-2897.
[5]	Huan-Feng TANG,Zai-Yin HUANG,Ming XIAO. Effects of Particle Size and Temperature on Surface Thermodynamic Functions of Cubic Nano-Cu₂O [J]. Acta Phys. -Chim. Sin., 2016, 32(11): 2678-2684.
[6]	GUO Zheng, HU Xin-Gen, LIANG Hong-Yu, JIA Zhao-Peng, CHENG Wei-Na, LIU Jia-Min. Enthalpic Pairwise Interactions of α-Aminobutyric Acid Enantiomers in DMF+Water Mixtures [J]. Acta Phys. -Chim. Sin., 2012, 28(09): 2015-2022.
[7]	HAN Yu-Mei, XU Xiu-Ying, ZHANG Shao-Feng, YAN Dan, XIAO Xiao-He, PENG Cheng. Microcalorimetric Investigation of Effect of Toad Bufanolide Diene on Tetrahymena Thermophila BF5 Growth [J]. Acta Phys. -Chim. Sin., 2010, 26(06): 1693-1698.
[8]	TANG Hui-Ying, YAN Dan, ZHANG Qing-Zhe, ZHANG Shao-Feng, XIAO Xiao-He, LIU Rong-Hua. Microcalorimetric Investigation of Four Bioactive Extracts from Cornu Cervi Pantotrichumon Intestinal Diagnostic Flora Growth [J]. Acta Phys. -Chim. Sin., 2010, 26(05): 1442-1447.
[9]	MI Yan, HUANG Zai-Yin, JIANG Jun-Ying, LI Yan-Fen. In situ Microcalorimetry Insight into the Growth of CaMoO₄ Microcrystallites [J]. Acta Phys. -Chim. Sin., 2009, 25(12): 2422-2426.
[10]	HU Xin-Gen, ZHU Yu-Qing, YU Sheng, ZHANG He-Juan, LIU Fei, YU Li. Aromatic π-π Self-Stacking of Some Aromatic Amino Acids in Aqueous Solutions [J]. Acta Phys. -Chim. Sin., 2009, 25(04): 729-734.
[11]	LIU Peng; LI Xi; PAN Mu. Binding Thermodynamic Constants of Alkaline Earth Metal Ions with 18-Crown-6 Studied by Microcalorimetry [J]. Acta Phys. -Chim. Sin., 2008, 24(01): 161-164.
[12]	HU Xin-Gen;YU Li;LIN Rui-Sen;FANG Ying-Ying;LI Wen-Bing . Study on Hydrophobic Self-association of Aliphatic α-amino Acids by Flow Microcalorimetry [J]. Acta Phys. -Chim. Sin., 2006, 22(08): 1034-1039.
[13]	LIU Jing; XU Gui-ying; LIU Jun; YU Li; ZHANG Hong-lin; YU Xiu-fang. Interaction between Tween-20 and BSA [J]. Acta Phys. -Chim. Sin., 2005, 21(08): 862-866.
[14]	Wang Shi-Bing;Song Ming-Zhi;Wei Xi-Lian;Yin Bao-Lin;Sun De-Zhi. A Study on Interactions between α-cyclodextrin and a Series of New Kind of Surfactants in Aqueous Solutions by Microcalorimetry [J]. Acta Phys. -Chim. Sin., 2004, 20(08): 837-842.
[15]	Liu Yi;Huang Yu-Ping;Gao Zhen-Ting;Duan Zhen-Hong;Shen Ping;Qu Song-Sheng. Promoter Function of Halobacterium halobium Chromosome DNA Fragment in Escherichia coli [J]. Acta Phys. -Chim. Sin., 2003, 19(09): 800-804.

Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Recommended 0