基于柔性衬底的ZnO葡萄糖酶电极制备及特性

doi:10.3866/PKU.WHXB201204062

Acta Phys. -Chim. Sin. ›› 2012, Vol. 28 ›› Issue (06): 1393-1397.doi: 10.3866/PKU.WHXB201204062

• ELECTROCHEMISTRY AND NEW ENERGY • Previous Articles Next Articles

Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

LI Jin-Hua^1,2, LI JING¹, FANG Xuan^1,2, WANG Xiao-Hua¹, WEI Zhi-Peng¹

1. School of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China;
2. International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology, Changchun 130022, P. R. China

Received:2012-03-09 Revised:2012-04-06 Published:2012-05-17
Contact: LI Jin-Hua E-mail:lijinhua2000@yahoo.com.cn
Supported by:
The project was supported by the National Natural Science Foundation of China (61006065, 61076039, 10804071), Research Fund for the Doctoral Program of Higher Education of China (20102216110001, 20112216120005), Developing Project of Science and Technology of Jilin Province, China (20091039, 20090555, 20101546, 20121816，201201116), and Foundation of Department of Education of Jilin Province, China (JYT2011-05, JYT2011-10, JYT2011-11).

Abstract

Abstract: Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase (GOx). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). GOx was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the GOx and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and GOx can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry.

Key words: Glucose oxidase, Flexible substrate, Biosensor, ZnO

LI Jin-Hua, LI JING, FANG Xuan, WANG Xiao-Hua, WEI Zhi-Peng. Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate[J]. Acta Phys. -Chim. Sin. 2012, 28(06), 1393-1397. doi: 10.3866/PKU.WHXB201204062

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Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

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