Acta Phys. -Chim. Sin. ›› 2006, Vol. 22 ›› Issue (07): 868-872.doi: 10.3866/PKU.WHXB20060720

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Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture

LIU Hui-Hong;WAN Yong-Qing;CHEN Xian-Tang;ZOU Guo-Lin   

  1. Department of Chemistry & Biology, Xiangfan University, Xiangfan 441053, P. R. China; College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China
  • Received:2006-01-16 Revised:2006-03-05 Published:2006-06-27
  • Contact: LIU Hui-Hong E-mail:liuhh@xfu.edu.cn

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Abstract: Horseradish peroxidase (HRP) was immobilized on glassy carbon electrode (GCE) surface by konjac glucomannan (KGM). HRP entrapped in the KGM film underwent fast direct transfer-electron reactions in water-organic solvent mixtures. The electrocatalysis of peroxides, such as hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and 2-butanone peroxide, by the immobilized HRP was also explored. Stability and reproducibility of the modified electrode made it possible to determine the organic peroxides.

Key words: Horseradish peroxidase, Electrochemistry, Peroxide