Acta Phys. -Chim. Sin. ›› 1999, Vol. 15 ›› Issue (10): 930-937.doi: 10.3866/PKU.WHXB19991013

• ARTICLE • Previous Articles     Next Articles

Study of β-1,4-glucosidase in Water/PC/Heptane-Butanol Reverse Micelles

Miao Wei, Yao Song-Nian   

  1. Center of Analysis and Measurement Wuhan University,Wuhan 430072
  • Received:1998-10-22 Revised:1998-12-15 Published:1999-10-15
  • Contact: Yao Song-Nian


 The maximum solubilization of water, acetic buffer and salicin in H 2O/PC/Heptan Butanol reverse micelles were measured. The change of structure of β-1,4-glucosidase and salicin in this system was investigated by UV ,FT IR and NMR. The activity and kinetics of β-1,4-glucosidase in this system were also studied. The results indicated that β-1,4-glucosidase follows the Michaelis-Menten kinetics in this reverse micelles, the v max is 1.475mg•min -1 ,11.8 times larger than that in aqueous, the Km is 0.389mg•mL -1 only 1/25 of that in aqueous. The reasons which cause this phenomenon are that the interaction at the interface of the reverse micelles changes the structure of β-1,4-glucosidase ,increases the concentration of salicin ,and that the -N +(CH 3) 3 in PC′ polar head attacks the oxygen of the glycoside bond of salicin .

Key words: β-1, 4-glucosidase, Phosphatidalcholine(PC), Reverse micelles, Solubilization, Activity