Acta Phys. -Chim. Sin. ›› 2002, Vol. 18 ›› Issue (07): 581-584.doi: 10.3866/PKU.WHXB20020702

• Communication • Previous Articles     Next Articles

Single Crystal Silicon Surface as a Novel Substrate of Gene-chip

Sun Bin;Wu Wei;Hong Lian;Zhao Xin-Sheng   

  1. State Key Laboratory of Molecular Dynamic and Stable Structures,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing　100871
  • Received:2002-03-18 Revised:2002-04-18 Published:2002-07-15
  • Contact: Zhao Xin-Sheng E-mail:zhaoxs@chem.pku.edu.cn

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Abstract: Single strand oligodeoxynucleotide was covalently attached to the alkyl modified silicon (111) surface through ester condensation reaction and the result was characterized by fluorescent spectra.The wafers modified by ss-oligodeoxynucleotide were used as DNA probes and the reusability of the resultant wafer was investigated.Its perfect stability in target detection process may promote the wider and more efficient application of the gene chip technology.

Key words: Gene-chip, Silicon(111) surface, Oligodeoxynucleotide, Ester condensation reaction, Covalent immobilization, Reusability