Acta Phys. -Chim. Sin. ›› 1995, Vol. 11 ›› Issue (04): 331-336.doi: 10.3866/PKU.WHXB19950409

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Laser Light Scattering Investigation of the Aggregation Process of Trichosanthin in Aqueous Solution

Wu Pei-Qiang,Ma Xing-Qi,Wu Qi   

  1. Department of Chemistry,Peking University,Beijing 100871|Institute of Biophysics,Academia Sinica,Beijing 100101|Department of Chemistry,The Chinese University of Hong Kong
  • Received:1994-06-01 Revised:1994-08-08 Published:1995-04-15
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Abstract:

Trichosanthin (TCS) is a single-chain protein containing 247 amino acid residues, it belongs to the plant ribosome-inactivating protein (RIP). The isoelectric point is about 9.4 and the mole mass is about 2.7×104 g•mol-1. It is an effective plant protein of a popular chinese herb medicine and a kind of cytotoxin. Recently, it has been reported that it can be used to inhibit trophoblastic tumors and may also be useful in the treatment of AIDS disease, and therefore has attracted moe attention to the RIP and its applications. However, the problem encoutered is its low stability in blood and tissue fluids. It has become essential to understand the behaviour of TCS in solution.

Key words: Laser light scattering, Trichosanthin, Aggregation process