物理化学学报 >> 2000, Vol. 16 >> Issue (05): 398-401.doi: 10.3866/PKU.WHXB20000504

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核苷酸碱基堆积导致的分子间NOE交叉峰

姜凌, 毛希安   

  1. 中国科学院武汉物理与数学研究所 波普于原子分子物理国家重点实验室，武汉 430071
  • 收稿日期:2000-01-03 修回日期:2000-03-08 发布日期:2000-05-15
  • 通讯作者:

Base-Stacking-Induced Intermolecular NOE Cross Peaks for Nucleotides

Jiang Ling, Mao Xi-an   

  1. Laboratory of NMR and Atomic Molecular Physics,Wuhan Institute of Physics Mathematics,Chinese Academy of Sciences,Wuhn 430071
  • Received:2000-01-03 Revised:2000-03-08 Published:2000-05-15
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关键词: 核苷酸, 碱基堆积, 二维NOE谱(NOESY)

Abstract:

The concept of base stacking of nucleotides in solutions has been well accepted. However, direct evidence has not been found. Here we report for the first time the intermolecular NOE cross peaks between ATP (adenosine 5’-triphosphate) and GTP (guanosine 5’-triphosphate), which unambiguously indicates the base stacking of nucleotides in solutions. In 400 mmol•L -1 ATPGTP solutions, the base protons of ATP, AH2 and AH8, show NOE cross peaks with the sugar protons of GTP, GH1′,GH2′ and GH3′.Also there are NOE cross peaks between the base proton of GTP (GH8) and the sugar protons of ATP. Possible conformations of base stacking in solutions have been discussed, and the distance between the stacked bases is estimated.

Key words: Nucleotide, Base stacking, NOESY