物理化学学报 >> 1992, Vol. 8 >> Issue (04): 523-526.doi: 10.3866/PKU.WHXB19920419

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固定HCG抗体膜的跨膜电位

吕鸣祥; 方道斌; 张杰; 陈昆明   

  1. 天津大学应用化学系，天津 300072；天津医学院
  • 收稿日期:1991-02-27 修回日期:1991-07-23 发布日期:1992-08-15
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The Transmembrane Potential of HCG Antibody-Binding Membrane

Lv Ming-Xiang; Fang Dao-Bin; Zhang Jie; Chen Kun-Ming   

  1. Department of Applied Chemistry, Tianjin University, Tianjian 300072; Tianjin Medical College
  • Received:1991-02-27 Revised:1991-07-23 Published:1992-08-15
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关键词: 人绒毛膜促性腺素(HCG), 跨膜电位, 兔疫化学反应

Abstract: In this paper the antibody of HCG was immobilzed on the cellulose acetate membrane. The membrane potentials varied with the electrolyte concentrations of the two aqueous phases. The charge density of the membrane phase was lowered as the immunochemical reaction of HCG with HCG antibody proceeded on the membrane surface. When c_1/c_2=8.0, the antibody-bound membrane potentials shifted to posi-tive values with the increase of HCG concentrations. The membrane immunosensor for the measurement HCG in the solution was constructed according to the change of the membrane potential. A linearity between Δφ and lg HCG can be obtained in the concentration range of 50—2500 mlU·ml~(-1) HCG.

Key words: Human chorionic gonadotropin(HCG), Transmembrane potential, Immunochemical reaction