关键词: 低能离子, 腺苷酸, 前生物合成, 高效液相色谱（HPLC）

Abstract: Low energy ion implantation has been widely used for mutation breeding,DNA delivery and microbe improvement et al.since 1980′s,but for a long time such kind of implantation was performed only to dry samples because it needed a vacuum chamber,for this reason,a new apparatus (see Fig.1)was designed in this paper to induce chemical reactions by introducing low energy ions into aqueous solution.In order to explore the origin of life induced by low energy ions and prebiotic synthesis of nucleotide via reaction between low energy ions and aqueous solution under primitive earth conditions,low energy N＋ was implanted into aqueous solution containing adenine,D ribose,and NH4H2PO4.HPLC analysis showed that a new substance was synthesized which was eluted as 5′AMP (to see Fig.2),then it was separated from the sample and analyzed by 1HNMR spectrum.It was confirmed that 5′AMP was formed in the experiment by 1HNMR spectrum (see Fig.3).The relation between yield of 5′AMP and irradiated time was obtained (see Fig.4).Mechanism of the production of 5′AMP was also discussed.The result is interesting for the formation and accumulation of nucleotide in the primitive oceans on the early earth.

Key words: Low energy ions, Adenosine-5′-monophosphate, Prebiotic synthesis, HPLC