物理化学学报 >> 1995, Vol. 11 >> Issue (04): 331-336.doi: 10.3866/PKU.WHXB19950409

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激光光散射研究天花粉蛋白的聚集过程

吴佩强,马星奇,吴奇   

  1. 北京大学化学系|北京 100871|中国科学院生物物理所|北京 100101|香港中文大学化学系
  • 收稿日期:1994-06-01 修回日期:1994-08-08 发布日期:1995-04-15
  • 通讯作者:

Laser Light Scattering Investigation of the Aggregation Process of Trichosanthin in Aqueous Solution

Wu Pei-Qiang,Ma Xing-Qi,Wu Qi   

  1. Department of Chemistry,Peking University,Beijing 100871|Institute of Biophysics,Academia Sinica,Beijing 100101|Department of Chemistry,The Chinese University of Hong Kong
  • Received:1994-06-01 Revised:1994-08-08 Published:1995-04-15
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摘要：

运用激光光散射技术研究天花粉蛋白在水溶液及不同浓度KSCN水溶液中的聚集过程．KSCN的加入能提高天花粉在水溶液中的稳定性. KSCN浓度大于0．5mol•L－1时，天花粉溶液透明、稳定，溶液中天花粉以单个分子与聚集体两种形式存在．聚集体主要是由约120个天花粉分子组成，平均流体力学半径Rh值约为49nm，在溶液中排列疏松，类似θ溶剂中的无规线团。

关键词: 激光光散射, 天花粉蛋白, 聚集过程

Abstract:

Trichosanthin (TCS) is a single-chain protein containing 247 amino acid residues, it belongs to the plant ribosome-inactivating protein (RIP). The isoelectric point is about 9.4 and the mole mass is about 2.7×104 g•mol-1. It is an effective plant protein of a popular chinese herb medicine and a kind of cytotoxin. Recently, it has been reported that it can be used to inhibit trophoblastic tumors and may also be useful in the treatment of AIDS disease, and therefore has attracted moe attention to the RIP and its applications. However, the problem encoutered is its low stability in blood and tissue fluids. It has become essential to understand the behaviour of TCS in solution.

Key words: Laser light scattering, Trichosanthin, Aggregation process