物理化学学报 >> 2010, Vol. 26 >> Issue (01): 18-22.doi: 10.3866/PKU.WHXB20100119

化学动力学和分子动态学 上一篇    下一篇

时间分辨拉曼光谱研究一氧化氮与肌红蛋白的结合过程

李涛, 吕荣, 于安池   

  1. 中国人民大学化学系, 北京 100872
  • 收稿日期:2009-09-14 修回日期:2009-10-27 发布日期:2009-12-29
  • 通讯作者: 于安池 E-mail:a.yu@chem.ruc.edu.cn

Recombination Process between Myoglobin and NO Studied by Time-Resolved Raman Spectroscopy

LI Tao, LV Rong, YU An-Chi   

  1. Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China
  • Received:2009-09-14 Revised:2009-10-27 Published:2009-12-29
  • Contact: YU An-Chi E-mail:a.yu@chem.ruc.edu.cn

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摘要：

纳秒瞬态拉曼光谱技术是研究分子结构变化超快动态过程的重要实验手段之一. 而肌红蛋白(Mb)与小分子配体的结合过程一直是人们研究的焦点. 本文旨在利用纳秒瞬态拉曼光谱技术研究小分子配体NO与肌红蛋白结合的动力学过程. 通过考察MbNO光解后产物脱氧肌红蛋白(DeoxyMb)与反应物MbNO的υ4特征振动峰的强度比值随激光激发功率的变化, 阐述了利用纳秒瞬态拉曼光谱技术研究MbNO体系中NO与DeoxyMb结合过程的可行性. 利用纳秒瞬态拉曼光谱技术, 获得了与皮秒时间分辨拉曼和皮秒时间分辨吸收相一致的结合动力学实验结果. 为研究其它复杂体系的超快结合动力学过程提供了一种新的思路.

关键词: 纳秒瞬态拉曼, 皮秒时间分辨拉曼, 皮秒时间分辨吸收, 肌红蛋白, 一氧化氮, 结合过程

Abstract:

Nanosecond transient Raman spectroscopy is a widely used technique to investigate ultrafast structural dynamics in molecules. We studied the rebinding kinetics between deoxymyoglobin (DeoxyMb) and NO using nanosecond transient Raman spectroscopy. By monitoring the ratio between the intensity of the υ4 vibrational mode in the photoproduct (DeoxyMb) and in the reactant (MbNO) at different incident laser fluxes, we obtained the recombination kinetics between DeoxyMb and NO in MbNO. For comparison, the kinetics of NO rebinding to DeoxyMb in MbNO was also studied using picosecond time-resolved Raman and absorption experiments. The kinetics results obtained with nanosecond transient Raman were consistent with those obtained by picosecond time-resolved Raman and absorption experiments.

Key words: Nanosecond transient Raman, Picosecond time-resolved Raman, Picosecond time-resolved absorption, Myoglobin, NO, Recombination