物理化学学报 >> 2026, Vol. 42 >> Issue (2): 100134.doi: 10.1016/j.actphy.2025.100134

所属专题： 碳点功能材料

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源自绿萝叶提取物的红光碳点制备和生物成像应用

邵仁义1, 库拉姆·阿巴斯2, 弗拉基米尔·尤里耶维奇·奥西波夫3, 朱海梅1, 李远1, 乌萨马1,*(), 毕红1,*()   

  1. 1 安徽大学材料科学与工程学院, 安徽 合肥 230601
    2 安徽大学化学化工学院, 安徽 合肥 230601
    3 Ioffe Institute, St. Petersburg 194021, Russian Federation
  • 收稿日期:2025-06-06 修回日期:2025-07-17 录用日期:2025-07-22 发布日期:2025-12-03
  • 通讯作者: Email: bihong@ahu.edu.cn (毕红)24743@ahu.edu.cn (乌萨马)

Red-emitting carbon dots prepared from Epipremnum Aureum leaves extract for biological imaging

Renyi Shao1, Khurram Abbas2, Vladimir Yu. Osipov3, Haimei Zhu1, Yuan Li1, Usama1,*(), Hong Bi1,*()   

  1. 1 School of Materials Science and Engineering, Anhui University, Hefei 230601, Anhui Province, China
    2 School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, Anhui Province, China
    3 Ioffe Institute, St. Petersburg 194021, Russian Federation
  • Received:2025-06-06 Revised:2025-07-17 Accepted:2025-07-22 Published:2025-12-03
  • Contact: Email: bihong@ahu.edu.cn (Hong Bi)24743@ahu.edu.cn (Usama)

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摘要：

荧光碳点(CDs)已经广泛应用于生物体外/体内的荧光成像。然而，荧光CDs作为生物标记的特异性较差、而且在红光发射区域荧光量子产率(QY)较低，仍然是亟待解决的关键问题。本研究以天然植物绿萝叶片的乙醇提取液为前驱体，通过溶剂热“一锅”法合成了一种红色荧光碳点(命名为EA-CDs，λex/λem = 400 nm/660 nm)。EA-CDs具有小尺寸(平均粒径3.9 nm)、高荧光QY (在乙醇中λem = 660 nm，QY = 15.4%)、低毒性(体外/体内)和良好的亲脂性(油水分离系数LogP > 0)，因此适用于体外/体内生物荧光成像和标记。实验结果表明该红光CDs不仅可以用于标记植物细胞膜，而且可以作为斑马鱼体内肠道荧光成像工具，有望成为一种通用型具有高荧光量子产率的生物膜红光染料。此外，本工作也为绿萝等观赏性植物的应用开拓了一种新思路。

关键词: 碳点, 红光发射, 生物成像, 膜染料, 绿萝, 斑马鱼

Abstract:

Carbon dots (CDs) have been widely applied in fluorescence imaging both in vitro and in vivo. However, key challenges remain to be addressed, including the poor specificity of CDs as biological markers and their relatively low fluorescence quantum yield (QY) in the red emission region. In this study, we synthesized red fluorescent carbon dots (designated as EA-CDs, λex/λem = 400 nm/660 nm) using a natural plant-derived precursor ethanol extract from Epipremnum aureum leaves via a one-pot solvothermal method. The EA-CDs exhibit a small particle size (average diameter: 3.9 nm), high fluorescence QY (15.4% in ethanol at λem = 660 nm), low toxicity (both in vitro and in vivo), and favorable lipophilicity (oil-water partition coefficient LogP > 0), making them suitable for biological fluorescence imaging and labeling applications. Experimental results indicate that these red-emitting CDs can not only effectively label plant cell membranes, but also serve as an intestinal fluorescence imaging probe in zebrafish models. This suggests their potential as a universal red-emissive bio-membrane dye with high QY. Furthermore, this work pioneers a novel application approach for ornamental plants like Epipremnum aureum.

Key words: Carbon dots, Red emission, Biological imaging, Membrane dyes, Epipremnum aureum, Zebrafish