Abstract: The development of human genome project calls for more rapid and accurate protein structure prediction method to assign the structure and function of gene products. Threading has been proved to be successful in protein fold assignment,although difficulties remain for low homologous sequences. We have developed a method for solving the sequence rearrangement problem in threading. By reshuffling secondary elements,protein structures with the same spatial arrangement of secondary structures but different connections can be predicted. This method has been proved to be useful in fold recognition for proteins of different evolutionary origin and converge to the same fold.

Key words: Protein structure prediction, Fold recognition, Low homology, 　Convergent evolution, Genome