Acta Phys. -Chim. Sin. ›› 2004, Vol. 20 ›› Issue (02): 202-206.doi: 10.3866/PKU.WHXB20040220

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Characterization of Interaction between Gatifloxacin and Bovine Serum Albumin

Guo Ming;Yan Jian-Wei;Yu Qing-Sen;Shang Zhi-Cai;Lü Jian-De   

  1. Department of Chemistry, Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 310027
  • Received:2003-06-21 Revised:2003-09-15 Published:2004-02-15
  • Contact: Yu Qing-Sen

Abstract: The present study describes the application of capillary zone electrophoresis(CZE) to investigate the interaction between bovine serum albumin and gatifloxacin, the simplified method of Hummel-Dreyer was applied and the experimental condition was established after a large number of tests. Based on the site-binding model, practical formula for small ligand molecule to bio-macromolecule has been proposed; the binding parameters were measured according to suggested model by non-linear fitting program.

Key words: Gatifloxacin, Bovine serum albumin(BSA), Capillary electrophoresis,  Non-linear fitting