BSA与羟磷灰石相互吸附的FTIR-ATR光谱

doi:10.3866/PKU.WHXB201511301

Abstract

Abstract:

The microcosmic process of bovine serum albumin (BSA) adsorbing onto hydroxyapatite (HA) for different time intervals was investigated by Fourier transform infrared attenuated total internal reflectance (FTIRATR) spectrometry. The initial dissolution and re-precipitation of PO₄^3-, Ca²⁺, and OH^- ions from the HA coating led to the occurrence of the coating including adsorbed BSA on the HA from surface-to subsurface-molecular layers and to in-depth interaction between BSA and HA. The subtraction results gained in the adsorption regions of HA and BSA reveal that the binding of P=O, from the phosphate (PO₄^3-), to the hydrogen of amide II, methyl and methene of the BSA appears to be considerably more rapid and stronger than that of the P―O group. In addition, it is very likely that Ca²⁺ plays an important role in the interaction of BSA with HA. It appears that the binding of Ca²⁺ to the carbonyl-oxygen of the peptide bond in BSAcaused a significant, molecular, conformational rearrangement of polypeptide backbones from β-pleated sheet to helical circles of α-helix and β-turn. This change appears to have been followed by much hydrogen of polypeptides being driven to bind PO₄^3- and OHeffectively and much ―C=O and H―N―groups of the peptide bond being freed from inter-chain hydrogenbonding to act on Ca²⁺ and combine strongly with the HA surface. This might reasonably be expected to promote hard tissue regeneration. BSA seems to be activated by the inductive effect of Ca²⁺ via the molecular rearrangement of polypeptide backbones from pleated sheet to helical circles and in turn reacts strongly on the HA, resulting in profound effects on the course of biomineralization.

Key words: Biomaterial, Protein adsorption, Polypeptide, IR spectroscopy, FTIR-ATR, Biomineralization

Qing YE,Ren HU,Jian-Zhang ZHOU,Yi-Wen YE,Zhao-Xi XU,Chang-Jian LIN,Zhong-Yu LIN. FTIR-ATR Spectrometry of BSA Adsorption on Hydroxyapatite[J]. Acta Phys. -Chim. Sin. 2016, 32(2), 565-572. doi: 10.3866/PKU.WHXB201511301

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FTIR-ATR Spectrometry of BSA Adsorption on Hydroxyapatite

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