季铵盐型阳离子表面活性剂与牛血清白蛋白的相互作用

doi:10.3866/PKU.WHXB201609231

Abstract

Abstract:

UV-visible (UV-Vis) absorption spectroscopy, fluorescence spectroscopy (FL), dynamic light scattering (DLS) and isothermal titration calorimetry (ITC) were used to study the interactions between bovine serum albumin (BSA) and the three quaternary ammonium surfactants N-dodecyl-N-(2-hydroxyethyl)-N, Ndimethyl ammonium bromide (DHDAB), N-tetradecyl-N-(2-hydroxyethyl)-N, N-dimethyl ammonium bromide (THDAB) and N-cetyl-N-(2-hydroxyethyl)-N, N-dimethyl ammonium bromide (CHDAB). These surfactants quenched the intrinsic fluorescence of BSA, with longer alkyl chains resulting in more significant quenching. This was attributed to static quenching. Further evidence of static quenching was provided by UV-Vis absorption spectroscopy. The particle size of BSA was found to initially increase and then decrease with increasing surfactant concentration. The concentration of surfactant changed the type of interaction mode. This work revealed the mechanism and binding characteristics between surfactants and protein, and provides the basis for further applications of surfactants.

Key words: Surfactants, Bovine serum albumin, Fluorescence quenching, Dynamic light scattering, Isothermal titration calorimetry

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Hu-Jun XIE,Cheng-Cheng LIU,Qiang SUN,Qing GU,Qun-Fang LEI,Wen-Jun FANG. The Interactions between Quaternary Ammonium Cationic Surfactants and Bovine Serum Albumin[J]. Acta Phys. -Chim. Sin. 2016, 32(12), 2951-2960. doi: 10.3866/PKU.WHXB201609231

References

1	Fainerman V. B. ; Zholob S. A. ; Leser M. ; Michel M. ; Miller R. J. Colloid Interface Sci. 2004, 274, 496.
2	Gull N. ; Chodankar S. ; Aswal V. K. ; Sen P. ; Khan R. H. Colloids Surf. B: Biointerfaces 2009, 69, 122.
3	Mehta S. K. ; Bhasin K. K. ; Kumar A. Colloids Surf. A: Physicochem. Eng. Aspects 2009, 346, 195.
4	Turro N. J. ; Lei X. G. ; Ananthapadmanabhan K. P. ; Aronson M. Langmuir 1995, 11, 2525.
5	Mehan S. ; Aswal V. K. ; Kohlbrecher J. Langmuir 2014, 30, 9941.
6	Mackie A. ; Wilde P. Adv. Colloid Interface Sci. 2005, 117, 3.
7	Chi E. Y. ; Krishnan S. ; Randolph T.W. ; Carpenter J. F. Pharm. Res. 2003, 20, 1325.
8	De S. ; Girigoswami A. ; Das S. J. Colloid Interface Sci. 2005, 285, 562.
9	Miller R. ; Fainerman V. B. ; Makievski A. V. ; Kr?gel J. ; Grigoriev D. O. ; Kazakov V. N. ; Sinyachenko O. V. Adv. Colloid Interface Sci. 2000, 86, 39.
10	Coke M. ; Wilde P. J. ; Russell E. J. ; Clark D. C. J. Colloid Interface Sci. 1990, 138, 489.
11	McCormack F. X. ; King T. E. ; J r. ; Voelker D. R. ; Robinson P.C. ; Mason R. J. American Review of Respiratory Disease 1991, 144, 160.
12	Bordbar A. K. ; Taheri-Kafrani A. Colloids Surf. B: Biointerfaces 2007, 55, 84.
13	Wang Y. ; Guo R. ; Xi J. J. Colloid Interface Sci. 2009, 331, 470.
14	Gelamo E. L. ; Tabak M. Spectrochim. Acta. A 2000, 56, 2255.
15	Kwaambwa H. M. ; Maikokera R. Colloids Surf. B: Biointerfaces 2008, 64, 118.
16	Klajnert B. ; Bryszewska M. Bioelectrochemistry 2002, 55, 33.
17	Carvalho C. M. L. ; Cabral J. M. S. Biochimie 2000, 82, 1063.
18	Rozema D. ; Gellman S. H. J. Am. Chem. Soc. 1995, 117, 2373.
19	Al-Shakhshir R. H. ; Regnier F. E. ; White J. L. ; Hem S. L. Vaccine 1995, 13, 41.
20	Ahmad A. L. ; Hairul N. A. H. Sep. Purif. Technol. 2009, 66, 273.
21	Gentili P. L. ; Ortica F. ; Favaro G. J. Phys. Chem. B 2008, 112, 16793.
22	Deep S. ; Ahluwalia J. C. Phys. Chem. Chem. Phys. 2001, 3, 4583.
23	Madaeni S. S. ; Rostami E. Chem. Eng. Technol. 2008, 31, 1265.
24	Zhao L. ; Liu R. ; Zhao X. ; Yang B. ; Gao C. ; Hao X. ; Wu Y. Sci. Total. Environ 2009, 47, 5019.
25	Reynolds J. A. ; Herbert S. ; Polet H. ; Steinhardt J. Biochemistry 1967, 6, 937.
26	Mehta S. K. ; Bhasin K. K. ; Kumar A. J. Colloid Interface Sci. 2008, 323, 426.
27	Lissi E. ; Abuin E. ; Lanio M. E. ; Alvarez C. J. Biochem. Biophys. Methods 2002, 50, 261.
28	Wang Y. Q. ; Zhang H. M. ; Zhang G. C. ; Tao W. H. ; Tang S. H. J. Lumin. 2007, 126, 211.
29	Gauthier T. D. ; Shane E. C. ; Guerin W. F. ; Seitz W. R. ; Grant C. L. Environ. Sci. Technol. 1986, 20, 1162.
30	Marras S. A. ; Kramer F. R. ; Tyagi S. Nucleic. Acids. Res. 2002, 30, 122.
31	Fraiji L. K. ; Hayes D. M. ; Werner T. C. J. Chem. Educ. 1992, 69, 424.
32	Lakowicz J. R. ; Weber G. Biochemistry 1973, 12, 416.
33	Gray J. J. Curr. Opin. Struc. Biol. 2004, 14, 110.
34	Seetharamappa J. ; Kamat B. P. Chem. Pharm. Bul. 2004, 52, 1053.
35	Eftink M. R. ; Ghiron C. A. Anal. Biochem. 1981, 114, 199.
36	Ware W. R. J. Phys. Chem. 1962, 66, 455.
37	Papadopoulou A. ; Green R. J. ; Frazier R. A. J. Agric. Food. Chem. 2005, 53, 158.
38	Barik A. ; Priyadarsini K. I. ; Mohan H. Photochem. Photobiol. 2003, 77, 597.
39	Congdon R.W. ; Muth G.W. ; Splittgerber A. G. Anal. Biochem. 1993, 213, 407.
40	Martin V. I. ; Rodriguez A. ; Maestre A. ; Moya M. L. Langmuir 2013, 29, 7629.
41	Zhang Y. Z. ; Zhou B. ; Liu Y. X. ; Zhou C. X. ; Ding X. L. ; Liu Y. J. Fluoresc. 2008, 18, 109.
42	Hu Y. J. ; Liu Y. ; Jiang W. ; Zhao R. M. ; Qu S. S. J. Photochem. Photobiol. B. 2005, 80, 235.
43	Zhou T. ; Ao M. ; Xu G. ; Liu T. ; Zhang J. J. Colloid Interface Sci. 2012, 389, 175.
44	Ojha B. ; Das G. Chem. Phys. Lipids 2011, 164, 144.
45	Jaiswal S. ; Mondal R. ; Paul D. ; Mukherjee S. Chem. Phys. Lett. 2016, 646, 18.
46	Ró?ycka-Roszak B. ; Wo?niak E. ; Misiak P. ; Fr?ckowiak R. ; Wilk K. A. J. Chem. Thermodyn. 2013, 66, 1.
47	Covis R. ; Vives T. ; Gaillard C. ; Benoit M. ; Benvegnu T. Carbohydrate Polymers 2015, 121, 436.
48	Xiang J. ; Fan J. B. ; Chen N. ; Chen J. ; Liang Y. Colloids Surf. B: Biointerf. 2006, 49, 175.
49	Asker D. ; Weiss J. ; McClements D. J. Langmuir 2009, 25, 116.
50	Bordbar A. K. ; Taheri-Kafrani A. ; Mousavi H. A. ; Haertle T. Arch. Biochem. Biophys. 2008, 470, 103.
51	Misra P. K. ; Dash U. ; Maharana S. Colloids Surf. A: Physicochem. Eng. Aspects 2015, 483, 36.

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The Interactions between Quaternary Ammonium Cationic Surfactants and Bovine Serum Albumin

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