Acta Phys. -Chim. Sin. ›› 1999, Vol. 15 ›› Issue (02): 173-177.doi: 10.3866/PKU.WHXB19990215

• ARTICLE • Previous Articles     Next Articles

The application of ESR-spin Stable Technique in Laccase Chemical System

Hua Jian-Li, Song Cai-Sheng, Wang Guang-Hui, Cai Ming-Zhong   

  1. Department of Chemistry,Jiangxi Normal University,Nanchang 330027|College of Chemistry,Wuhan University,Wuhan 430072
  • Received:1998-04-20 Revised:1998-08-25 Published:1999-02-15
  • Contact: Hua Jian-Li

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Abstract:

A method for the effective generation of high steady state concentration of semiquinone radicals was established in laccase/O2 system by using ESR-spin stable technique in static condition. The result of ESR demonstrated that the laccase-catalyzed reaction of these substrates underwent the stage of semiquinones.

Key words: Rhus vernicifeva laccase, Semiquinone radical, Catechol substrates, ESR-spin stable technique