Acta Phys. -Chim. Sin. ›› 2000, Vol. 16 ›› Issue (12): 1073-1079.doi: 10.3866/PKU.WHXB20001204

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Interaction between Actin and Cisplatin or Transplatin

Yang Yong, Duan Li-Li, Li Zhi-Fen, Zeng Hui-Hui, Wang Bao-Huai, Zhang You-Min   

  1. Institute of Physical Chemistry,Peking University 100871|School of Pharmaceutical Science,Beijing Medical University,Beijing 100083
  • Received:2000-03-15 Revised:2000-06-05 Published:2000-12-15
  • Contact: Li Zhi-Fen E-mail:lyjzf@pku.edu.cn

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Abstract:

The interaction between actin and cisplatin or transplatin hes been studied by using the 1H-NMR spectra and measuring the critical concentration of actin polymerization.These studies show that both cisplatin and transplatin induce a depolymerization of F-actin,and that the effect of transplatin is more obvious than that of cisplatin at the same concentration.It is found that cisplatin promotes a polymerization of G-actin at low concentration and inhibits it at high concentration.while transplatin inhibits the polymerization at any concentration.The binding sites of cisplatin/transplatin to G-actin are discussen.It is probable that cisplatin binds with Cys-10 and Cys-285,while tracsplatin does not bind with Cys-10.

Key words: Actin, Cisplatin, Transplatin, 1H-NMR