基于低分子量聚乙烯亚胺和油酸的负电性基因载体

doi:10.3866/PKU.WHXB201311282

Abstract

Abstract:

The development of synthetic vectors based on low-molecular-weight polyethylenimine (PEI) and the construction of negatively charged vectors with high nucleic acid encapsulation are two of the current research trends in gene delivery. In the present study, we developed a stable and negatively charged lipopolyplex based on oleic acid and a low-molecular-weight PEI (2 kDa); it was formed by simultaneously mixing oleic acid micelles and a polyplex produced from PEI (2 kDa) and oligo dsDNA. This lipopolyplex is stable in serum, and because of the low molecular weight of the PEI, the lipopolyplex exhibits very low toxicity. The encapsulation efficiencies of nucleic acids by traditional anionic vectors are low, but very high (more than 80%) by this lipopolyplex. Suitable surface modification with 1,2-distearoryl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethyleneglycol-2000) ammonium salt enabled the lipopolyplex to bind to cells, and a high cellular uptake efficiency (ca 90%) was observed in vitro.

Key words: Polyethylenimine, Oleic acid, Gene delivery

Click here to download Supporting Info material in PDF type

YANG Yang, GUO Xia. Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid[J]. Acta Phys. -Chim. Sin. 2014, 30(2), 345-350. doi: 10.3866/PKU.WHXB201311282

References

(1) Xu, L.; Anchordoquy, T. J. Pharmaceutical Sci. 2011, 100,38. doi: 10.1002/jps.22243
(2) Malam, Y.; Lim, E. J.; Seifalian, A. M. Current Medicinal Chemistry 2011, 18, 1067. doi: 10.2174/092986711794940860
(3) Guo, X.; Huang, L. Accounts of Chemical Research 2012, 45,971. doi: 10.1021/ar200151m
(4) Boussif, O.; Lezoualc′h, F.; Zanta, M. A.; Mergny, M. D.;Scherman, D.; Demeneix, B.; Behr, J. P. Proc. Natl. Acad. Sci. U. S. A. 1995, 92, 7297. doi: 10.1073/pnas.92.16.7297
(5) Godbey,W. T.; Barry, M. A.; Saggau, P.;Wu, K. K.; Mikos, A.G. J. Biomed. Mater. Res. 2000, 51, 321.
(6) Bonner, D. K.; Zhao, X.; Buss, H.; Langer, R.; Hammond, P. T.J. Control. Release 2013, 167, 101. doi: 10.1016/j.jconrel.2012.09.004
(7) Mahato, M.; Kumar, P.; Sharma, A. K. Molecular Biosystems2013, 9, 780. doi: 10.1039/c3mb25444e
(8) Patnaik, S.; Gupta, K. C. Expert Opinion on Drug Delivery2013, 10, 215. doi: 10.1517/17425247.2013.744964
(9) Ogris, M.;Walker, G.; Blessing, T.; Kircheis, R.;Wolschek, M.;Wagner, E. J. Control. Release 2003, 91, 173. doi: 10.1016/S0168-3659(03)00230-X
(10) Urban-Klein, B.;Werth, S.; Abuharbeid, S.; Czubayko, F.;Aigner, A. Gene Therapy 2005, 12, 461. doi: 10.1038/sj.gt.3302425
(11) Li, S.;Wang, Y.; Zhang, J.; Yang,W. H.; Dai, Z. H.; Zhu,W.;Yu, X. Q. Mol. Biosyst. 2011, 7, 1254. doi: 10.1039/c0mb00339e
(12) Kunath, K.; Harpe, A. V.; Fischer, D.; Petersen, H.; Bickel, U.;Voigt, K.; Kissel, T. J. Control. Release 2003, 89, 113. doi: 10.1016/S0168-3659(03)00076-2
(13) Tsai, L. R.; Chen, M. H.; Chien, C. T.; Chen, M. K.; Lin, F. S.;Lin, K. M. C.; Hwu, Y. K.; Yang, C. S.; Lin, S. Y. Biomaterials2011, 32, 3647. doi: 10.1016/j.biomaterials.2011.01.059
(14) Chen, J.; Tian, H.; Guo, Z.; Xia, J.; Kano, A.; Maruyama, A.;Jing, X.; Chen, X. Macromol. Biosci. 2009, 9, 1247. doi: 10.1002/mabi.v9:12
(15) Zhang, L.; Lu, Z.; Zhao, Q.; Huang, J.; Shen, H.; Zhang, Z.Small 2011, 7, 460. doi: 10.1002/smll.201001522
(16) Werth, S.; Urban-Klein, B.; Dai, L.; Höbel, S.; Grzelinski, M.;Bakowsky, U.; Czubayko, F.; Aigner, A. J. Control. Release2006, 112, 257. doi: 10.1016/j.jconrel.2006.02.009
(17) Jiang, Q.; Shi, P.; Li, C.;Wang, Q.; Xu, F.; Yang,W.; Tang, G.Macromol. Biosci. 2011, 11, 435. doi: 10.1002/mabi.v11.3
(18) Roesler, S.; Koch, F. P. V.; Schmehl, T.;Weissmann, N.; Seeger,W.; Gessler, T.; Kissel, T. J. Gene Med. 2011, 13, 123. doi: 10.1002/jgm.v13.2
(19) Alshamsan, A.; Haddadi, A.; Incani, V.; Samuel, J.; Lavasanifar,A.; Uludag, H. Molecular Pharmaceutics 2009, 6, 121. doi: 10.1021/mp8000815
(20) Boura, J. S.; Santos, F. D.; Gimble, J. M.; Cardoso, C. M. P.;Madeira, C.; Cabral, J. M. S.; Silva, C. L. D. Human Gene Therapy Methods 2013, 24, 38. doi: 10.1089/hgtb.2012.185
(21) Morigaki, K.;Walde, P. Current Opinion in Colloid & Interface Science 2007, 12, 75. doi: 10.1016/j.cocis.2007.05.005
(22) Ferreira, D. A.; Bentley, M. V. L. B.; Karlsson, G.; Edwards, K.Int. J. Pharm. 2006, 310, 203. doi: 10.1016/j.ijpharm.2005.11.028
(23) Edwards, K.; Silvander, M.; Karlsson, G. Langmuir 1995, 11,2429. doi: 10.1021/la00007a020
(24) Wang, B.; Rouzier, R.; Albarracin, C. T.; Sahin, A.;Wagner, P.;Yang, Y.; Smith, T. L.; Meric-Bernstam, F.; Marcelo, A. C.;Hortobagyi, G. N.; Pusztai, L. Breast Cancer Research and Treatment 2004, 87, 205. doi: 10.1007/s10549-004-6590-0
(25) Vader, P.; Aa, L. J. v. d.; Engbersen, J. F. J.; Storm, G.;Schiffelers, R. M. J. Control. Release 2010, 148, 106. doi: 10.1016/j.jconrel.2010.06.019

[1]	Ye Fan, Chongmei Cao, Yun Fang, Yongmei Xia. Fabrication of Fluorescent Nanodots by Self-Crosslinking Ufasomes of Conjugated Linoleic Acid and Their Unique Fluorescence Properties [J]. Acta Phys. -Chim. Sin., 2022, 38(3): 2002032-.
[2]	Shaohai LI,Bo WENG,Kangqiang LU,Yijun XU. Improving the Efficiency of Carbon Quantum Dots as a Visible Light Photosensitizer by Polyamine Interfacial Modification [J]. Acta Phys. -Chim. Sin., 2018, 34(6): 708-718.
[3]	Han WANG,Xiao-Min WANG. Effect of Preparation Conditions on the Optical Properties of PEI-Functionalized Graphene Quantum Dots [J]. Acta Phys. -Chim. Sin., 2016, 32(5): 1267-1272.
[4]	Peng-Cheng BIAN,Da-Peng ZHANG,Hong-Ze GANG,Jin-Feng LIU,Bo-Zhong MU,Shi-Zhong YANG. Synthesis and Properties of a Novel Bio-Based Branched Heptadecylbenzene Sulfonate Derived from Oleic Acid [J]. Acta Phys. -Chim. Sin., 2016, 32(11): 2753-2760.
[5]	MA Jie, FAN Ye, FANG Yun. Influence of Alkyl Polyglucoside on Migration and Extension of pH Window for Forming Fatty Acid Vesicles of Conjugated Linoleic Acid [J]. Acta Phys. -Chim. Sin., 2015, 31(7): 1359-1364.
[6]	YAO Man-Li, DONG Yan-Yan, XIE Jing, JIA Ai-Ping, XIE Guan-Qun, HU Geng-Shen, LUO Meng-Fei. Preparation of Polyethylenimine-Functionalized Silica Nanotubes and Their Application for CO₂ Adsorption [J]. Acta Phys. -Chim. Sin., 2014, 30(4): 789-796.
[7]	YAO Er-Gang, ZHAO Feng-Qi, GAO Hong-Xu, XU Si-Yu, HU Rong-Zu, HAO Hai-Xia, AN Ting, PEI Qing, XIAO Li-Bai. Thermal Behavior and Non-Isothermal Decomposition Reaction Kinetics of Aluminum Nanopowders Coated with an Oleic Acid/Hexogen Composite System [J]. Acta Phys. -Chim. Sin., 2012, 28(04): 781-786.
[8]	SHAO Yu; DAI Wen-xin; WANG Xu-xu; DING Zheng-xin; LIU Ping; FU Xian-zhi. The Photocatalytic Property of Anodized Al2O3 Film on Al Surface [J]. Acta Phys. -Chim. Sin., 2005, 21(06): 622-626.
[9]	He Zhan-Bo;Li Dong-Mei;Shi Yu-Quan. Soap Liquid Membrane Oscillators [J]. Acta Phys. -Chim. Sin., 2002, 18(11): 979-984.
[10]	Dai Le-Rong. The Structure of Liquid Crystal and Solubilization of Cholesterol in Lecithin-Water-Oleic Acid System [J]. Acta Phys. -Chim. Sin., 1996, 12(02): 159-162.
[11]	Liu Mu-Xin.Xu Gui-Ying.Li Gan-Zuo.Mao Hong-Zhi.Li Fang. The Transient Interfacial Tension between Oleic Acid-sodium Oleate Aqueous Solution and Crude Oil [J]. Acta Phys. -Chim. Sin., 1995, 11(11): 1040-1043.

Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid

PDF

Like

Knowledge

Abstract

Supporting Info

Cite this article

share this article

References

Related Articles 11

Metrics

Recommended 0