Acta Phys. -Chim. Sin. ›› 1993, Vol. 9 ›› Issue (05): 702-705.doi: 10.3866/PKU.WHXB19930525

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Pulse Radiolysis of Rabbit Liver Metallothionein in Aqueous Solution

Fang Xing-Wang; Li Feng-Mei; Wu Ji-Lan; Di Shao-Jie; Wei Gen-Shuan; Ru Bing-Gen   

  1. Department of Technical Physics, Peking University, Beijing 100871; Institute of Law Energy, Beijing Normal University, Beijing 100875; Life Science Center, Peking University Beijing 100871
  • Received:1992-03-09 Revised:1992-08-15 Published:1993-10-15
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Abstract: Metallothioneins(MTs) are widely occuring proteins characterizied by a low molecular weight (6500—6800) and an extremely high metal and sulfur content. The mechanizm of pulse radiolysis of rabbit liver MT-I in aqueous solution was studied. After pulse radiolysis, two peaks occured with maximum absorption at 450 nm and 280 nm. It was supposed that the first peak was due to RSSR~- radical formed by the combination of RS·radical and RS~- anion. The observed kinetic constants of growth and decay of RSSR~- radical were estimated from the absorption spectra.

Key words: Metallothionein, Pulse radiolysis, Radiolysis mechanizm