Acta Phys. -Chim. Sin. ›› 2008, Vol. 24 ›› Issue (08): 1359-1365.doi: 10.3866/PKU.WHXB20080806

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Mechanism of Photokilling LoVo Cells by Nano TiO2 Photocatalytic Oxidation Combined with Immunity and Electroporation Method

SUN Yi; XU Juan; CAI Wen-Bin; JIANG Zhi-Yu   

  1. Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, P. R. China; Department of Chemical Engineering, Jiangsu Polytechnic University, Changzhou 213164, Jiangsu Province, P. R. China
  • Received:2008-03-03 Revised:2008-04-14 Published:2008-08-06
  • Contact: CAI Wen-Bin; JIANG Zhi-Yu E-mail:zyjiang@fudan.ac.cn;wbcai@fudan.edu.cn

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Abstract: The nano TiO2 photocatalytic oxidation method combined with immunity and electroporation techniques performed high efficiency for photokilling LoVo cancer cells even at very low concentration (3.12 μg·mL-1). In this method, the LoVo cells could be killed completely within 30 min under the irradiation of UV light (wavelength 253.7 nm, 4 mW·cm-2). The mechanism of this process was investigated using confocal florescent microscopy, TEM, and comet assay methods. The results demonstrated that the antibody modified nano TiO2 particles could adsorb on the cell membrane, after electroporation it could enter the inside of LoVo cells and located around the karyon. Under the irradiation of UV light, the cell’s structure, organelle, and DNA were destroyed seriously, which caused the necroses of LoVo cells. Because the photocatalysis effect of nano TiO2 took place inside of cells, so the photokilling efficiency enhanced strongly.

Key words: Nano TiO2, LoVo cancer cell, Photocatalytic oxidation, Immunity, Electroporation, Comet assay