物理化学学报 >> 1991, Vol. 7 >> Issue (04): 471-474.doi: 10.3866/PKU.WHXB19910418

研究简报 上一篇    下一篇

微量热法测定细菌的临界生长温度

谢昌礼; 汤厚宽; 宋昭华; 屈松生; 廖耀庭; 刘海水   

  1. 武汉大学化学系，武汉 430072；广州军区武汉总医院
  • 收稿日期:1990-03-02 修回日期:1990-10-04 发布日期:1991-08-15
  • 通讯作者: 谢昌礼

Critical Growth Temperatures of Bacteria by Microcalorimetric Method

Xie Chang-Li; Tang Hou-Kuan; Song Zhao-Hua; Qu Song-Sheng; Liao Yao-Ting; Liu Hai-Shui   

  1. Department of Chemistry, Wuhan University, Wuhan 430072; Army Hospital of Guangzhou Military Area, Wuhan
  • Received:1990-03-02 Revised:1990-10-04 Published:1991-08-15
  • Contact: Xie Chang-Li

PDF

2752

赞

关键词: 微量量热学, 生长热谱, 临界生长温度

Abstract: In this paper, we have determined the multiplication rate constanis (k) of Shigella flexneri and E. coli. at different temperatures and in different culture media by means of a microcalorimeter. From these results a linear equation as k~(1/2)=a+bT can be established for the bacterial growth. The corresponding linear equation for S. flexneri is k~(1/2)=-1.2823+0.0047122 T(r=0.971), so its eritica lgrowth temperature T_0 is equal to 272.1 K. The linear equation for E coli is k~(1/2)=-1.6390+0.005948 T (r=0.994), T_0=275.5 K. The experimental results indicate that the critial growth temperature of E. coli. is nearly a constant (T_0=273.9 K) in different culture media, which is very informative for the study on microorganism growth.

Key words: Microcalorimetry, Growth thermogram, Critial growth temperature