物理化学学报 >> 1994, Vol. 10 >> Issue (04): 354-359.doi: 10.3866/PKU.WHXB19940413

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介体型酶电极二级催化反应速度常数的测定

吕鸣祥, 戴洪礼, 张国衡   

  1. 天津大学应用化学系，天津 300072
  • 收稿日期:1992-09-15 修回日期:1993-03-20 发布日期:1994-04-15
  • 通讯作者:

The Determination of the Second Order Rate Coefficient of Enzymatic Reaction for the Mediator Enzyme Electrode

Lv Ming-Xiang, Dai Hong-Li, Zhang Guo-Heng   

  1. Department of Applied Chemistry, Tianjin University, Tianjin 300072
  • Received:1992-09-15 Revised:1993-03-20 Published:1994-04-15
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摘要：

采用开路驰豫法，测定了以二茂铁及其衍生物作为电子传递体的介体型酶电极的均相二级催化反应速度常数，结果表明二甲氨基甲基二茂铁，在绝氧条件下对于酶的再生反应具有高的催化活性，是一种性能优良的电子传递体．此外，讨论了开路弛豫法在酶电极研究中的适用条件及各种因素对该方法测定结果的影响．

关键词: 酶电极, 介体, 开路弛豫, 速度常数

Abstract:

In this paper, the second order rate coefficient for homogeneous catalytic re action of ferrocene and its derivative have been detendned by open circult relaxation diator in redox enzyme catalysis for the anaerobic oxidation of glucose catalyzed by glucose oxidase. Moreover, the feasibility and conditions for the application of the open circuit relaxation method is discussed.

Key words: Enzyme electrode, Mediator, Open circuit relaxation, Rate coefficient