物理化学学报 >> 1999, Vol. 15 >> Issue (02): 173-177.doi: 10.3866/PKU.WHXB19990215

研究论文 上一篇    下一篇

ESR自旋稳定化技术在漆酶化学中的应用

花建丽, 宋才生, 王光辉, 蔡明中   

  1. 江西师范大学化学系，南昌 330027|武汉大学化学学院，武汉 430072
  • 收稿日期:1998-04-20 修回日期:1998-08-25 发布日期:1999-02-15
  • 通讯作者: 花建丽

The application of ESR-spin Stable Technique in Laccase Chemical System

Hua Jian-Li, Song Cai-Sheng, Wang Guang-Hui, Cai Ming-Zhong   

  1. Department of Chemistry,Jiangxi Normal University,Nanchang 330027|College of Chemistry,Wuhan University,Wuhan 430072
  • Received:1998-04-20 Revised:1998-08-25 Published:1999-02-15
  • Contact: Hua Jian-Li

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摘要：

用ESR自旋稳定化技术对十六个儿茶酚类底物的漆酶催化氧化反应进行了ESR追踪，捕获到相应的半醌自由基．结果表明，ESR自旋稳定化技术的运用，开辟了一条在静态条件下用漆酶／O2体系获取高稳态浓度半醌自由基的有效途径．

关键词: 漆树漆酶, 半醌自由基, 几茶酚类底物, ESR自旋稳定化技术

Abstract:

A method for the effective generation of high steady state concentration of semiquinone radicals was established in laccase/O2 system by using ESR-spin stable technique in static condition. The result of ESR demonstrated that the laccase-catalyzed reaction of these substrates underwent the stage of semiquinones.

Key words: Rhus vernicifeva laccase, Semiquinone radical, Catechol substrates, ESR-spin stable technique