物理化学学报 >> 2010, Vol. 26 >> Issue (04): 840-849.doi: 10.3866/PKU.WHXB20100429

胶体及界面化学 上一篇    下一篇

荧光探针技术在水溶液两亲分子有序组合体研究中的应用

赵莉, 阎云, 黄建滨   

  1. 北京大学化学与分子工程学院, 分子动态与稳态结构国家重点实验室, 北京 100871
  • 收稿日期:2009-11-17 修回日期:2010-01-04 发布日期:2010-04-02
  • 通讯作者: 阎云, 黄建滨 E-mail:jbhuang@pku.edu.cn; yunyan@pku.edu.cn

Application of Fluorescent Probe Technology in Self-Assembly Systems of Amphiphile Aqueous Solutions

ZHAO Li, YAN Yun, HUANG Jian-Bin   

  1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
  • Received:2009-11-17 Revised:2010-01-04 Published:2010-04-02
  • Contact: YAN Yun, HUANG Jian-Bin E-mail:jbhuang@pku.edu.cn; yunyan@pku.edu.cn

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摘要：

总结了荧光探针技术在两亲分子有序组合体研究中的应用. 在两亲分子有序组合体研究中, 荧光技术的应用非常广泛. 尤其是荧光探针技术的应用, 为两亲分子有序组合体微观信息的获取提供了一个简单便利的渠道. 根据荧光探针分子的荧光光谱, 如最大发射波长、荧光强度及荧光寿命等参数, 可以得到临界聚集浓度、微粘度、微极性等信息; 根据探针的荧光猝灭, 尤其是时间分辨荧光猝灭技术, 可探测有序组合体的聚集数、聚集数变化相应的聚集体转化及表面电荷等信息. 近年来发展起来的含荧光基团的两亲分子因其可以对聚集体微环境进行原位检测而引起了了广泛的关注. 本文对上述几个方面的相关工作分别作了介绍.

关键词: 荧光探针, 猝灭, 有序组合体, 两亲分子, 聚集体转变, 表面活性剂

Abstract:

In this paper, we review the application of fluorescent probe technology in self-assembly systems of amphiphile aqueous solutions. Fluorescence technology, especially fluorescence probe technology has been extensively employed to explore local information about various molecular assemblies. The following contents are covered: (1) The critical aggregate concentration, microviscosity, and micropolarity may be obtained fromfluorescence parameters such as emission maxima, fluorescence intensity, and lifetime etc. (2) Probe quenching, especially, time-resolved fluorescence quenching (TRFQ) can give information about aggregation number and surface charge density which can be used as the indication on aggregate transition. (3) Fluorophore-labeled amphiphiles which can take part in the formation of aggregates reveal more precise information, so that an in situ investigation of local environments is possible. Moreover, fluorescence resonance energy transfer (FRET) and excited state proton transfer (ESPT) are also useful in this field. Therefore, fluorescence probe technology provides us with a simple and effective method to study organized amphiphiles systems.

Key words: Fluorescent probe, Quenching, Organized assembly, Amphiphile, Aggregate transition, Surfactant