物理化学学报 >> 2011, Vol. 27 >> Issue (06): 1467-1473.doi: 10.3866/PKU.WHXB20110606

软物质 上一篇    下一篇

Gemini表面活性剂与DNA在气/液界面上的相互作用

康雪丽, 陈启斌, 尚亚卓, 刘洪来   

  1. 华东理工大学, 结构可控先进功能材料及其制备教育部重点实验室, 上海 200237
  • 收稿日期:2010-11-18 修回日期:2011-03-05 发布日期:2011-05-31
  • 通讯作者: 陈启斌 E-mail:qibinchen@ecust.edu.cn
  • 基金资助:

    国家自然科学基金(20806025, 20706013), 长江学者创新团队(IRT0721), 教育部留学回国人员科研启动基金和中央高校基本科研业务费资助

Interaction between Gemini and DNA at the Air/Water Interface

KANG Xue-Li, CHEN Qi-Bin, SHANG Ya-Zhuo, LIU Hong-Lai   

  1. Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237, P. R. China
  • Received:2010-11-18 Revised:2011-03-05 Published:2011-05-31
  • Contact: CHEN Qi-Bin E-mail:qibinchen@ecust.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (20806025, 20706013), Creative Team Development Project of Ministry of Education of China (IRT0721), Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry and Fundamental Research Funds for the Central Universities, China.

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摘要：

在气/液界面上, 阳离子表面活性剂可以通过静电作用与阴离子型的脱氧核糖核酸(DNA)分子形成复合膜, 并压缩沉积得到LB(Langmuir-Blodget)膜. 利用表面压-表面积(π-A)曲线、原子力显微镜(AFM)和石英晶体微天平(QCM)研究了阳离子Gemini表面活性剂([C18H37(CH3)2N+-(CH2)s-N+(CH3)2C18H37]·2Br-, 简写为18-s-18, s=3, 4, 6, 8, 10, 12)与DNA(双链DNA(dsDNA), 单链DNA(ssDNA))之间的相互作用, 并对18-s-18在不同下相表面的分子面积进行了比较. 实验结果表明连接基团和下相的DNA对Gemini表面活性剂在气/液界面上的性质有很大影响. 此外, Gemini表面活性剂在界面上对DNA的吸附能力与它们之间的相互作用方式密切相关.

关键词: Gemini表面活性剂, 脱氧核糖核酸, π-A等温线, 原子力显微镜, 石英晶体微天平

Abstract:

Monolayers may be obtained by an electrostatic force between cationic surfactants and anionic electrolyte deoxyribonucleic acid (DNA) molecules, and a corresponding Langmuir-Blodgett (LB) complex monolayer can be fabricated by compression and deposition of the monolayer at the air/water interface. In this work, the interaction between cationic Gemini surfactants ([C18H37(CH3)2N+-(CH2)s-N+(CH3)2C18H37]·2Br-, abbreviated 18-s-18, s=3, 4, 6, 8, 10, 12) and double-strand DNA (dsDNA)/single-strand DNA (ssDNA) was investigated by surface pressure-surface area (π-A) isotherms, atomic force microscope (AFM), and Quartz crystal microbalance (QCM). Moreover, the limiting molecular areas of 18-s-18 on different subphases were compared. We found that the spacer and the subphase greatly influence the properties of the Gemini surfactants at the air/water interface. In addition, we conclude that the adsorption capacity of the Gemini surfactants with DNA is closely related to their interaction modes.

Key words: Gemini surfactant, Deoxyribonucleic acid, π-A isotherm, Atomic force microscope, Quartz crystal microbalance