Abstract: Using MS 80 standard microcalorimeter and specific measuring tube, we have obstained the normal growth thermal graphs of Hela cell and Human Andenocarcinoma Gastic cell (SGc 7901), the thermal graph of Hela cells synchronized in M phase of cell-cycle at the start and the themal graph of Gc cells in culture medium containing anticancer medicine, determined the relationships of the number of cells, the peak height of themal graph and the specific heat output power. In addition, our work has shown the prospects of microcalormetic method in cytological study.