物理化学学报 >> 1990, Vol. 6 >> Issue (03): 283-289.doi: 10.3866/PKU.WHXB19900307

研究论文 上一篇    下一篇

液相色谱双保留机理动力学过程研究

张玉奎; 邹汉法; 卢佩章   

  1. 中国科学院大连化学物理研究所；中国大连色谱技术研究开发中心
  • 收稿日期:1988-07-20 修回日期:1989-03-15 发布日期:1990-06-15
  • 通讯作者: 张玉奎

The Investigation of Dynamic Process of Daul Retention Mechanism in RP-HPLC

Zhang Yu-Kui; Zou Han-Fa; Lu Pei-Chang   

  1. Dalian Institute of Chemical Physica, Academia Sinica, Dalian; Chromatographic R D Center of China
  • Received:1988-07-20 Revised:1989-03-15 Published:1990-06-15
  • Contact: Zhang Yu-Kui

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摘要： 本文根据质量平衡原理建立了反相色谱双保留机理的质量平衡模型, 导出了色谱流出曲线的一阶矩和二、三阶中心矩, 获得了双保留机理色谱容量因子和塔片高度的表达式. 讨论了各种色谱动力学参数对柱效和峰形的影响, 对改善色谱峰对称度和提高柱效的措施作了理论说明。

Abstract: The mass balance model of daul retention mechanism in RP-HPLC is established based on the theory of mass transfer. The first moment and the second, third central moments are derived. The total capacity factor k' and HETP in daul retentio-mechanism can be expressed as:
k′=k′_1+k′_2
H=(2γD_m)/μ+2λa_o+{k[k_0+(1+k_0)(k'_1+k'_2)]~2d~(5/3)_pu~(2/3)}/{(1+k_0)~2(1+k'_1+k'_2)~2D~(2/3)_m}
+{θ[k_0+(1+k_0)(k'_1+k'_2)]~2d_pu}{30D_mk_0(1+k_0)~2(1+k'_1+k'_2)~2}+(2u)/{((1+k_0)(1+k'_1+k'_2)~2}(k'_1/k_(d1)+k'_2/k_(d2))
The effects of chromatographic parameters on column efficiency and peak shape are discussed. The approaches to increase column efficiency and eliminate the tail of chromatogram are explained.