物理化学学报 >> 2007, Vol. 23 >> Issue (01): 84-87.doi: 10.3866/PKU.WHXB20070117

研究论文 上一篇    下一篇

溶菌酶热变性的DSC研究

方盈盈;胡新根;于丽;李文兵;林瑞森   

  1. 温州大学化学与材料科学学院, 浙江 温州 325027; 山东大学胶体与界面化学教育部重点实验室, 济南 250100; 浙江大学化学系, 杭州 310027
  • 收稿日期:2006-06-02 修回日期:2006-08-21 发布日期:2007-01-08
  • 通讯作者: 胡新根 E-mail:hxgwzu@126.com

Study on Thermal Denaturation of Lysozyme by DSC

FANG Ying-Ying;HU Xin-Gen;YU Li;LI Wen-Bing;LIN Rui-Sen   

  1. School of Chemistry and Materials Science, Wenzhou University, Wenzhou 325027, Zhejiang Province, P. R. China; Key Laboratory for Colloid and Interface Chemistry of the Ministry of Education, Shandong University, Jinan 250100, P. R. China; Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China
  • Received:2006-06-02 Revised:2006-08-21 Published:2007-01-08
  • Contact: HU Xin-Gen E-mail:hxgwzu@126.com

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摘要： 用差示扫描量热法研究了固体溶菌酶的热变性以及水溶液中不同变性剂与浓度对溶菌酶变性的影响. 结果表明, 溶剂水的存在及变性剂尿素和盐酸胍的加入使溶菌酶的变性温度降低, 变性焓减小; 同时, 在一定的浓度范围内, 溶菌酶的变性温度和变性焓随变性剂浓度的增大而降低. 盐酸胍的变性效果较尿素强, 这是由于盐酸胍与蛋白质分子间除了氢键作用外还存在着静电作用.

关键词: 溶菌酶, 尿素, 盐酸胍, DSC, 热变性

Abstract: The thermal denaturation of solid lysozyme and the effects of denaturants and their concentrations on denaturation of lysozyme in aqueous solutions were studied by differential scanning calorimetry (DSC). The results showed that both denaturation temperature and denaturation enthalpy of lysozyme decreased as the existence of water and the addition of urea and guanidine hydrochloride (GuHCl). In addition, denaturation temperature and denaturation enthalpy decreased as the concentrations of urea and GuHCl increased. As a denaturant, GuHCl is more effective than urea due to its added ability of electrostatic interaction.

Key words: Lysozyme, Urea, GuHCl, DSC, Thermal denaturation