物理化学学报 >> 2007, Vol. 23 >> Issue (08): 1178-1182.doi: 10.3866/PKU.WHXB20070808

研究论文 上一篇    下一篇

表面活性剂与血红蛋白的作用对转移电子数的影响

高筱玲; 郭彦; 田燕妮; 赵健伟   

  1. 山西大学分子科学研究所, 太原 030006; 南京大学化学化工学院, 生命分析化学教育部重点实验室, 南京 210008
  • 收稿日期:2007-01-09 修回日期:2007-04-28 发布日期:2007-08-03
  • 通讯作者: 田燕妮; 赵健伟 E-mail:tianyann@sxu.edu.cn, zhaojw@nju.edu.cn

Influence of Surfactant on the Apparent Electron Transfer Number of Hemoglobin

GAO Xiao-Ling; GUO Yan; TIAN Yan-Ni; ZHAO Jian-Wei   

  1. Institute of Molecular Science, Shanxi University, Taiyuan 030006, P. R. China; Key Laboratory of Analytical Chemistry for Life Science of the Ministry of Education, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210008, P. R. China
  • Received:2007-01-09 Revised:2007-04-28 Published:2007-08-03
  • Contact: TIAN Yan-Ni; ZHAO Jian-Wei E-mail:tianyann@sxu.edu.cn, zhaojw@nju.edu.cn

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摘要： 将不同浓度(0.15-10.0 mmol·L-1)的双十二烷基二甲基溴化铵(DDAB)和血红蛋白(Hb)依次修饰到热解石墨电极(PG)上, 制备了稳定的Hb-DDAB/PG电极. 利用循环伏安和紫外光谱研究了Hb-DDAB/PG电极的性质. 由Laviron模型得到的转移电子数表明该电化学反应由双电子过程逐步过渡到单电子过程, 进而证明DDAB膜厚的增加导致血红蛋白亚基间作用的减弱和亚铁血红素的释放.

关键词: 血红蛋白, 双十二烷基二甲基溴化铵, 表面电化学, 转移电子数

Abstract: A modified electrode was prepared by sequential dropping didodecyldimethylammonium bromide (DDAB) with different concentrations and hemoglobin (Hb) solution on pyrolytic graphite (PG) electrode. The electrochemical property of the modified electrode was studied by cyclic voltammetry and UV spectrum. The data analyzed with Laviron model showed that with increasing the DDAB concentration, the thickness of films increased and the heme was released from hemoglobin, so the electrochemical reaction changed from a double-electron process to a single-electron process.

Key words: Hemoglobin, Didodecyldimethylammoniumbromide, Surface electrochemistry, Electron transfer number