物理化学学报 >> 2008, Vol. 24 >> Issue (03): 543-546.doi: 10.3866/PKU.WHXB20080334

研究简报 上一篇    

肉桂酸与牛血清白蛋白相互作用及酒精的影响

闫华; 杨健国; 梁华定; 潘富友; 赵松林; 李晶晶   

  1. 台州学院医药化工学院, 浙江 临海 317000; 台州学院设备管理处, 浙江 临海 317000
  • 收稿日期:2007-08-03 修回日期:2007-11-04 发布日期:2008-03-10
  • 通讯作者: 杨健国 E-mail:yjg@tzc.edu.cn

Interaction between Cinnamic Acid and Bovine Serum Albumin and the Influence of Alcohol

YAN Hua; YANG Jian-Guo; LIANG Hua-Ding; PAN Fu-You; ZHAO Song-Lin; LI Jing-Jing   

  1. School of Pharmaceutical and Chemical Engineering, Taizhou University, Linhai 317000, Zhejiang Province, P. R. China; Office of Assets Administration, Taizhou University, Linhai 317000, Zhejiang Province, P. R. China
  • Received:2007-08-03 Revised:2007-11-04 Published:2008-03-10
  • Contact: YANG Jian-Guo E-mail:yjg@tzc.edu.cn

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摘要： 用荧光光谱法研究了肉桂酸与牛血清白蛋白(BSA)在生理条件下的相互作用. 实验结果表明, 肉桂酸与BSA能形成1:1复合物, 荧光猝灭属于静态猝灭过程; 与BSA分子间主要的结合作用力为疏水作用; 310 K 下结合常数和结合位点数分别为3.07×104 L·mol-1和1.10; 肉桂酸使BSA的构象发生了变化; 另外, 酒精的加入使其结合常数和结合位点数减小.

关键词: 肉桂酸； 牛血清白蛋白； 酒精； 荧光光谱； 相互作用

Abstract: The interaction between cinnamic acid and bovine serumalbumin (BSA) has been studied with fluorimeter under physiological conditions. The experimental results demonstrated that the combination reaction of cinnamic acid and BSA was a static quenching process because of forming 1:1 complex, and the dominant binding force was hydrophobic interaction. At 310 K, the binding constant and the number of binding sites were 3.07×104 L·mol-1 and 1.10, respectively. And the conformation of BSA was changed due to the presence of cinnamic acid. Furthermore, the binding constant and the number of binding sites would decrease due to adding alcohol.

Key words: Cinnamic acid, Bovine serumalbumin, Alcohol, Fluorescence spectrum, Interaction