物理化学学报 >> 2007, Vol. 23 >> Issue (02): 237-241.doi: 10.3866/PKU.WHXB20070219

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一种含芴基的钌(II)配合物的合成及DNA键合性质

姚威;吴宝燕;高丽华;王科志   

  1. 北京师范大学化学系, 北京 100875;北京工商大学环境与化工学院, 北京 100037
  • 收稿日期:2006-08-07 修回日期:2006-09-06 发布日期:2007-02-01
  • 通讯作者: 王科志 E-mail:kzwang@bnu.edu.cn

DNA-Binding Studies of a Fluorenyl-Containing Ru(II) Complex

YAO Wei;WU Bao-Yan;GAO Li-Hua;WANG Ke-Zhi   

  1. Department of Chemistry, Beijing Normal University, Beijing 100875, P. R. China; 2School of Chemical and Environment Engineering, Beijing Technology and Business University, Beijing 100037, P. R. China
  • Received:2006-08-07 Revised:2006-09-06 Published:2007-02-01
  • Contact: WANG Ke-Zhi E-mail:kzwang@bnu.edu.cn

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摘要： 合成了一种新的钌(II)配合物[Ru(bpy)2(Hfip)](ClO4)2, 其中bpy代表2,2′-联吡啶, Hfip代表2-(9H-芴-2-基)-1H-咪唑-[4,5-f]-[1,10]-邻菲啰啉. 通过紫外可见光谱、荧光光谱、稳态荧光淬灭、与溴化乙锭的竞争实验、粘度测量和DNA热变性研究了该配合物与小牛胸腺DNA的键合性质. 结果表明, 该配合物能嵌入键合DNA, 键合常数Kb=8.6×105 L·mol-1 (50 mmol·L-1 NaCl).

关键词: 钌(II), DNA, 邻菲啰啉, 紫外可见光谱, 荧光光谱

Abstract: A ruthenium(II) complex of [Ru(bpy)2(Hfip)](ClO4)2 (bpy=2,2′-bipyridine, Hfip=2-(9H-fluoren-2-yl)-1H-imidazolo-[4,5-f]-[1,10]-phenanthrolin) was synthesized. The DNA binding properties of the complex were studied by means of electronic absorption and emission spectroscopic titrations, steady-state emission quenching by Fe(CN)4-6, viscosity measurements, DNA competitive binding with ethidium bromide, and thermal denaturation of DNA. The results indicated that the complex bound to calf-thymus DNA in an intercalative mode with a binding constant of 8.6×105 L·mol-1 in the presence of 50 mmol·L-1 NaCl solution.

Key words: Ruthenium (II), DNA, Phenanthroline, UV-Vis spectrum, Emission spectrum