金纳米粒子比色测定细胞色素c构象变化

doi:10.3866/PKU.WHXB20100923

Abstract

Abstract:

The colors of Au nanoparticles (Au NPs) change along with conformational changes in cytochrome c (Cyt c). We exploited this property for the colorimetric detection of Cyt c conformational changes induced by H⁺ and L-cysteine (L-Cys). We improved the conventional procedure for this detection. After the addition of Cyt c within different pH values, the Au NPs are either cyan, blue, purple or red. This indicates that the Au NPs can be applied to the rapid colorimetric detection of pH-induced conformational changes in Cyt c. At pH 7 the color of Au NPs changes from purple to blue and then cyan upon the addition of L-Cys, which suggests that the Au NPs can be used for the colorimetric detection of Cyt c conformational changes caused by interaction with L-Cys. The conformational changes of Cyt c were verified by circular dichroism (CD) spectroscopy. The relationship between the aggregation states and colors of the Au NPs after the addition of Cyt c was characterized by UV-Vis absorption spectroscopy and scanning electron microscopy (SEM).

Key words: Au nanoparticle, Colorimetric detection, Conformational changes of Cyt c, pH value, L-Cys

LI Na, ZHOU Jian-Zhang, LIN Ling-Ling, HAN Nan-Nan, LIN Zhong-Hua. Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c[J]. Acta Phys. -Chim. Sin. 2010, 26(09), 2468-2472. doi: 10.3866/PKU.WHXB20100923

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Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c

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