电化学和紫外-可见吸收光谱法研究pH和金纳米粒子对细胞色素c的影响

doi:10.3866/PKU.WHXB201203073

Abstract

Abstract: In this paper, gold nanoparticles (NPs) with an average size of (4.7 ± 0.6) nm, capped with mercaptopropionic acid (MPA) ligand, are prepared. The effect of pH and Au NPs on cytochrome c (Cyt c) is investigated by electrochemistry and UV-Vis absorption spectroscopy. UV-Vis absorption spectra indicate that the structures of Cyt c and Cyt c-Au NPs complex do not change appreciably between pH=7.5 and pH=3.0, but their Soret band positions change markedly at pH=2.0, indicating that low pH value induces a conformational change in Cyt c. Cyclic voltammetry (CV) result shows that Au NPs capped with MPA enhance electron transfer between Cyt c and the electrode. The data also reveals that the biocompatibility of Au NPs is improved when citric acid ligand is replaced with MPA. The change in pH value causes a change of peak currents in CV and a shift of peak potential. When pH value deviates from 7.0, levels of electroactive Cyt c decrease. Significant pH change induces irreversible denaturing of Cyt c. The pH at which Cyt c-Au NPs complex denatures completely is one unit higher than that of Cyt c. Combining the results from UV-Vis spectroscopy and CV, we find that addition of Au NPs makes adsorbing state Cyt c more vulnerable to pH.

Key words: Au nanoparticle, Cytochrome c, pH value, Cyclic voltammetry, UV-Vis absorption spectroscopy

WANG Yan-Yan, JIANG Yan-Xia, SUSHAAndrei, ROGACH Andrey, SUN Shi-Gang. Effect of pH and Au Nanoparticles on Cytochrome c Investigated by Electrochemistry and UV-Vis Absorption Spectroscopy[J]. Acta Phys. -Chim. Sin. 2012, 28(05), 1127-1133. doi: 10.3866/PKU.WHXB201203073

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Effect of pH and Au Nanoparticles on Cytochrome c Investigated by Electrochemistry and UV-Vis Absorption Spectroscopy

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