Abstract: Effective management of fungal keratitis (FK) urgently demands innovative strategies integrating potent antifungal activity with robust anti-inflammatory effects to overcome rising antifungal resistance and corneal penetration barriers, preventing corneal fibrosis and vision loss. Herein, we develop an ultrasmall molybdenum diselenide (MoSe₂) quantum dot eye drop (MSQD) as a stimuli-free bifunctional platform. The MSQD exhibits an ultra-low minimum inhibitory concentration (MIC) of 0.05 μg mL^-1 against Fusarium solani, approximately 235-fold lower than the conventional first-line agent voriconazole (Vor, MIC 11.76 μg mL^-1). The MSQD demonstrates rapid and complete fungicidal activity, achieving 100% cell elimination within 1 h by inducing intracellular redox collapse and irreversible membrane damage. Furthermore, the MSQD possesses intrinsic catalase (CAT)-mimetic activities, efficiently scavenging intracellular reactive oxygen species, alleviating inflammation, and promoting a pro-healing corneal microenvironment. In addition, the quantum dimensions of MSQD facilitate deep corneal penetration through transient cell junction modulation, thus ensuring efficient delivery to the infection sites. In a mouse FK model, MSQD-treated eyes achieve complete pathogen eradication and restore corneal clarity indistinguishable from healthy tissue, exhibiting great bioavailability and a superior therapeutic effect compared to Vor. Collectively, these multifaceted attributes establish stimuli-free MSQD as a novel, highly efficient and safe bifunctional nanoplatform with superior potency and excellent biocompatibility, holding great promise for comprehensive management of fungal infections.

Key words: MoSe₂ quantum dots, Eye drops, Fungal keratitis, Inflammation eliminating, Quantum confinement